Product Description

CytoForm® 300 consists of a 4.5 x 4.5 x 0.15 cm 3D printed scaffold made out of electrically conductive 3D Graphene (Dimension Inx®). It comes as a large sheet that can be readily cut or punched out to meet experimental needs.

The CytoForm® 300 scaffolds consist of ~75% Graphene particles, and ~25% polylactide-co-glycolide (PLG).

These scaffolds provide interconnected porosity (for vascularization), high bioactivity, easy handling, workability and high mechanical stability. These features make the substrates ideal for cell culture in bone and hard tissue regeneration.

The CytoForm®-300 scaffolds are ethanol washed prior to shipment, but are not considered sterile.

Every other layer is offset in the X and Y by 0.5mm to provide optimal cell-seeding surface area.

CytoForm®-300 is chemically stable in neutral pH, is flexible, but also has compressive/mechanical strength.

Scaffolds can be seeded with cells or cells can also be suspended in a pre-hydrogel material (such as Type I collagen).

Storage/Stability:

The product ships and is stored at room temperature.

Parameter, Testing, and Method CytoForm®-300 #5238
Scaffold Size 4.5 x 4.5 x 0.15 cm
Pattern 0-90° Offset
Porosity ~600 micron
Interlayer Spacing 0.3mm
Shelf Life Minimum of 6 months from date of receipt

Directions for Use

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Sterilization

It is recommended to cut or punch samples intended for the experiment prior to sterilization (ie. 4mm biopsy punch).

  1. Wash samples in 70% ethanol for 30-60 minutes.
  2. Rinse samples 3-5 times in sterile water or PBS.
  3. Remove excess liquid from scaffolds via dabbing on filter paper or sterilize cloth, or aspirating out excess liquid. Do not allow scaffolds to become completely dry prior to cell seeding.

 Cell Seeding Instructions

  1. Place wetted scaffolds into desired tissue culture plate.
  2. Dispense 10 uL of cell suspension onto the scaffold for a 4-5mm diameter scaffold (~50,000 cells).
  3. Allow cells to attach for 30-60 minutes prior to adding additional media.
  4. Slowly fill the cell culture well with media and incubate at 37°C

Cell Seeding Instructions Using Collagen Hydrogel

  1. Place wetted scaffolds into desired tissue culture plate.
  2. Add cells to a neutralized collagen solution (such as PureCol® EZ Gel).
  3. Slowly dispense the collagen/cell solution over the scaffold and allow the solution to fully permeate throughout the scaffold.
  4. Allow collagen to polymerize within the scaffold for 60-90 minutes at 37°C.
  5. Fill the well with cell culture medium until the scaffold is completely covered and continue incubating at 37°C.]

Product Certificate of Analysis

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Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.