Product Description

Advanced BioMatrix offers PhotoCol®, a purified methacrylated Type I bovine collagen kit. PhotoCol® provides native-like 3D collagen gels with the unique attributes to be prepared at various concentrations and crosslinked to provide various gel stiffness.                                            

The PhotoCol® kit consists of purified methacrylated Type I bovine collagen as the core component with other support reagents in the kit.  Table 1 provides a list of the kit components.                                        

Table 1:    

Item Catalog Number Package Size Storage Temperature
Methacrylated Collagen #5198 100 mg 2-10°C
20 mM Acetic Acid #5079 50 mL 2-30°C
Neutralization Solution #5205 10 mL Room Temperature
Irgacure Photoinitiator #5200 100 mg 2-10°C

 

The methacrylated Type I collagen is produced from telo-peptide intact bovine collagen where the collagen has been modified by reacting the free amines, primarily the ε-amines groups of the lysine residues as well as the a-amines groups on the N-termini.  > 20% of the total lysine residues of the collagen molecule have been methacrylated. The collagen is extracted from bovine hide and contains a high monomer content.                         

The collagen starting material was isolated from a closed herd and purified using controlled manufacturing processes. A bottle of 20 mM acetic acid solution is provided to solubilize the lyophilized methacrylated collagen at concentrations ranging from 3 to 8 mg/ml. The neutralization solution consists of an alkaline 10X phosphate buffered saline (PBS) solution which provides physiological salts and pH in the final mixture. The photoinitiator consists of Irgacure 2959 to be formulated in methanol (methanol not included) which allows UV crosslinking of the collagen at 365 nm.       

Parameter, Testing, and Method Methacrylated Collagen #5198
Sterilization Method Filtration
Extraction Method Acid - Telocollagen
Form Lyophilized Powder
Degree of Methacrylation >20%
Package Size 100 mg
Shelf Life Minimum of 6 months from date of receipt
Shelf Life After Reconstitution 2 Months

Collagen Purity - Silver Staining

>99%
Kinetic Gel Test (Minutes) <40
Gel Formation Tube Test (Minutes) < 40

Electrophoretic Pattern - Coomassie Blue

Characteristic
Sterility - USP modified No growth
Endotoxin - LAL <10.0 EU/mL
Source Bovine Hide
Hydrogel Young's Modulus E (Pa) Characteristic

           

Product Applications

PhotoCol® Methacrylated Collagen is a photocrosslinkable type I collagen for 3D cell culture and bioprinting.

As the most abundant protein in the body, collagen is a key component for cell culture. Collagen methacrylate can be used as a rapidly self-assembling type I collagen to form cross-linked hydrogels for tissue engineering[1]. It has been used with mesenchymal stem cells[2], fibroblasts, adipose derived stem cells, epithelial cells, and many more.

Collagen methacrylate is useful for forming scaffolds with varying degree of stiffness, by altering collagen concentration or the dose of UV-light exposure[2]. It is also being extensively characterized for its usefulness in 3D bioprinting (extrusion, inkjet, and photolithographic[3]).

PhotoCol® has been used for created 3D microniches[4], as well as for 3D bioprinting human corneas[5].

References:

  1. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4172302/

  2. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4243547/

  3. http://www.worldscientific.com/doi/abs/10.1142/S2339547817500091

  4. https://onlinelibrary.wiley.com/doi/abs/10.1002/adbi.201700237

  5. https://www.ncbi.nlm.nih.gov/pubmed/29772228

Product References

References for PhotoCol®:

Isaacson, A., Swioklo, S. & Connon, C. J. 3D bioprinting of a corneal stroma equivalent. Experimental Eye Research 173, 188–193 (2018).

Kathryn E. Drzewiecki et al, A thermoreversible, photocrosslinkable collagen bio-ink for free-form fabrication of scaffolds for regenerative medicine, TECHNOLOGY (2017). 

Drzewiecki, K. E. et al. Methacrylation Induces Rapid, Temperature-Dependent, Reversible Self-Assembly of Type-I Collagen. Langmuir 30, 11204–11211 (2014).

Gaudet, I. D. & Shreiber, D. I. Characterization of Methacrylated Type-I Collagen as a Dynamic, Photoactive Hydrogel. Biointerphases 7, 25 (2012).

Izadifar, Mohammad, et al. "UV-assisted 3D bioprinting of nanoreinforced hybrid cardiac patch for myocardial tissue engineering." Tissue Engineering Part C: Methods 24.2 (2018): 74-88.

Stoecklin, Celine, et al. "A New Approach to Design Artificial 3D Microniches with Combined Chemical, Topographical, and Rheological Cues." Advanced Biosystems (2018): 1700237.

Mazzocchi, Andrea, et al. "Optimization of collagen type I-hyaluronan hybrid bioink for 3D bioprinted liver microenvironments." Biofabrication (2018).

Nguyen, T. U., Watkins, K. E. & Kishore, V. Photochemically crosslinked cell‐laden methacrylated collagen hydrogels with high cell viability and functionality. Journal of Biomedical Materials Research Part A107,1541–1550 (2019).

Mazzocchi, A. et al.Pleural Effusion Aspirate for Use in 3D Lung Cancer Modeling and Chemotherapy Screening. ACS Biomaterials Science & Engineering5,1937–1943 (2019).

Chhetri, A. et al. Cell Culture and Coculture for Oncological Research in Appropriate Microenvironments. Current Protocols in Chemical Biology11,(2019).

Product Certificate of Analysis

No result for .

Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.