Product Description

Advanced BioMatrix offers PhotoGel® LAP Kit, a purified (sterility tested) methacrylated gelatin kit for photocrosslinkable hydrogels. PhotoGel® provides 3D tunable gels with the unique attributes to be prepared at various concentrations and photocrosslinked to provide various gel stiffness.
The PhotoGel® kit consists of purified methacrylated porcine gelatin and a LAP Photoinitiator (405 nm). The gelatin is type A 300 bloom.

Table 1:

Item Catalog Number Package Size Storage Temperature
Methacrylated Gelatin #5208 1 gram (2 x 500 mg) -20°C
LAP Photoinitiator #5269 100 mg 2-10°C


PhotoGel® is produced from methacrylated gelatin where the gelatin has been modified by reacting the free amines, primarily the ε-amines groups of the lysine residues as well as the a-amines groups on the N-termini. >75% of the total lysine residues of the gelatin molecule have been methacrylated. The photoinitiator consists of LAP to be formulated in 1X PBS or cell culture media which allows blue light photocrosslinking of the collagen at 405 nm.

For aseptic work, we recommend filtering the solubilized LAP through a 0.2 micron button filter, as the photoinitiator does not come sterile.

Parameter, Testing, and Method Methacrylated Gelatin #5208
Sterilization Method Filtration
Sterility - USP modified No growth
Form Lyophilized Powder
Package Size 1 gram (2 x 500 mg)
Storage Temperature -20°C
Shelf Life Minimum of 6 months from date of receipt
Degree of Methacrylation > 75%

Source

Type A, 300 Bloom, Porcine Gelatin

Hydrogel Young's Modulus E (Pa)

Characteristic

Directions for Use

Download the full Directions for Use PDF or continue reading below:

3D Hyrdogel Preparation:

Note: Employ aseptic practices to maintain the sterility of the product throughout the preparation and handling of the collagen and other solutions.

Note: The following instructions are for a 10% gelatin methacrylate solution. Recommended concentrations are 5-20%.

  1. Warm 10 mL of warm, sterile 1X PBS or 1X cell culture media to >60°C.
  2. Add the 10 mL’s of warmed solution to the amber vial containing 1 gram of lyophilized gelatin methacrylate.
  3. Mix on a shaker table or rotator plate until fully solubilized. Keep warm (>37°C) if possible (eg. place your rotator in an incubator) to help with full solubilization.
  4. Calculate the volume of photoinitiator to add by multiplying the volume of solubilized gelatin by 0.02. If the resulting number is 200 ul, for example, you will add 200 ul of LAP
  5. Solubilize the required amount of LAP (per step 4) at a concentration of 17 mg/ml in 1X PBS or cell culture media.
  6. Add the LAP to the gelatin solution and fully mix until solution is homogeneous.
  7. Add your cells to the gelatin/photoinitiator solution.
  8. Dispense your gelatin/photoinitiator/cell solution into the desired dish (ie. 6-well plate, 48-well plate).
  9. For photocrosslinking, place hydrogel directly under a 405 nm visible light crosslinking source.

Any excess material can be refrigerated and stored. The material will gel. Warm back up to >30°C for it to become liquid again. We recommend only adding photoinitiator to the amount of gelatin to be used at that time.

Product Applications

PhotoGel® Gelatin methacrylate can be used to form cross-linked hydrogels for tissue engineering[1] and 3D printing. The common forms of 3D printing using Lifeink® 300 include extrusion[2][3][4], inkjet[5] and photolithography[6]).

PhotoGel® has been used for endothelial cell morphogenesis,[7] cardiomyocytes,[8] epidermal tissue[9], injectable tissue constructs[10], bone differentiation[11], and cartilage regeneration[12].

Gelatin methacrylate has been explored in drug delivery applications in the form of microspheres[13] and hydrogels[14].

References:

  1. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2878615/
  2. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4040163/
  3. https://www.ncbi.nlm.nih.gov/pubmed/24112804
  4. https://www.ncbi.nlm.nih.gov/pubmed/20387987
  5. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5380738/
  6. https://www.ncbi.nlm.nih.gov/pubmed/28349897/
  7. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3643201/
  8. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551408/
  9. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4608855/

Product References

References for PhotoGel®:

 

Rothrauff, Benjamin B., et al. "Efficacy of thermoresponsive, photocrosslinkable hydrogels derived from decellularized tendon and cartilage extracellular matrix for cartilage tissue engineering." Journal of tissue engineering and regenerative medicine 12.1 (2018): e159-e170.

Rothrauff, Benjamin B., et al. "Anatomical region-dependent enhancement of 3-dimensional chondrogenic differentiation of human mesenchymal stem cells by soluble meniscus extracellular matrix." Acta biomaterialia 49 (2017): 140-151.

Rothrauff, Benjamin B., Guang Yang, and Rocky S. Tuan. "Tissue-specific bioactivity of soluble tendon-derived and cartilage-derived extracellular matrices on adult mesenchymal stem cells." Stem cell research & therapy 8.1 (2017): 133.

Bridge, Jack Christopher, et al. "Electrospun gelatin-based scaffolds as a novel 3D platform to study the function of contractile smooth muscle cells in vitro." Biomedical Physics & Engineering Express 4.4 (2018): 045039.

Capella-Monsonís, Héctor, et al. "Scaffolds for tendon tissue engineering." Handbook of Tissue Engineering Scaffolds: Volume One. Woodhead Publishing, 2019. 259-298.

Product Certificate of Analysis

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Safety and Documentation

Safety Data Sheet

Certificate of Origin

Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.