-
Collagen
-
Type I - Atelocollagen
- PureCol® Solution, 3 mg/ml (bovine) #5005
- Nutragen® Solution, 6 mg/ml (bovine) #5010
- FibriCol® Solution, 10 mg/ml (bovine) #5133
- PureCol® EZ Gel, Solution, 5 mg/ml (bovine) #5074
- PureCol® Lyophilized, 15 mg (bovine) #5006
- VitroCol® Solution, 3 mg/ml (human) #5007
- VitroCol® Lyophilized, 15 mg (human) #5008
-
Type I - Telocollagen
- TeloCol®-3 Solution, 3 mg/ml (bovine) #5026
- TeloCol®-6 Solution, 6 mg/ml (bovine) #5225
- TeloCol®-10 Solution, 10 mg/ml (bovine) #5226
- RatCol® for 3D gels, Solution, 4 mg/ml (rat) #5153
- RatCol® High Concentration, Solution, 10 mg/ml (rat)
- RatCol® lyophilized, 100 mg (rat)
- RatCol® for Coatings, Solution, 4 mg/ml (rat) #5056
- Type I - Insoluble Collagen
- Type I - Bioinks
- Type II Collagen
- Type III Collagen
- Type IV Collagen
- Collagen Standard
- PureCol® Collagen Coated Plates
- Collagen Scaffolds
- Collagen Hybridizing Peptides
-
Type I - Atelocollagen
- Tunable Stiffness
- CytoSoft® Rigidity Plates
-
Bioprinting
- Support Slurry for FRESH Bioprinting
- Collagen Bioinks for Extrusion Bioprinting
- GelMA Bioinks for Extrusion Bioprinting
- Bioink Components
- Photoinitiators
- Methacrylated Collagen
- Methacrylated Gelatin
- Methacrylated Hyaluronic Acid
- HyStem® Thiolated Hyaluronic Acid
- Diacrylates
- Methacrylated Polysaccharides
-
3D Hydrogels
- Thermoreversible Hydrogel
- Silk Fibroin
-
Type I Collagen for 3D Hydrogels
- PureCol® Solution, 3 mg/ml (bovine) #5005
- Nutragen® Solution, 6 mg/ml (bovine) #5010
- FibriCol® Solution, 10 mg/ml (bovine) #5133
- PureCol® EZ Gel, Solution, 5 mg/ml (bovine) #5074
- VitroCol® Solution, 3 mg/ml (human) #5007
- TeloCol®-3 Solution, 3 mg/ml (bovine) #5026
- TeloCol®-6 Solution, 6 mg/ml (bovine) #5225
- TeloCol®-10 Solution, 10 mg/ml (bovine) #5226
- RatCol® for 3D gels, Solution, 4 mg/ml (rat) #5153
- HyStem® Thiolated Hyaluronic Acid
- Methacrylated Collagen
- Methacrylated Gelatin
- Methacrylated Hyaluronic Acid
- Diacrylates
- Collagen Sponges
- Methacrylated Polysaccharides
- Extracellular Matrices
- HyStem / Hyaluronic Acid
-
Adhesion Peptides / Proteins
-
Recombinant Adhesion Proteins
- CD2, 0.5 mg/ml #5086
- CDH3, 0.5 mg/ml #5124
- CDH13, 0.5 mg/ml #5125
- CD14, 0.5 mg/ml #5089
- CDH18, 0.5 mg/ml #5090
- CD40, 0.5 mg/ml #5093
- CD86, 0.5 mg/ml #5096
- CD164, 0.5 mg/ml #5100
- CD270, 0.5 mg/ml #5127
- CD274, 0.5 mg/ml #5126
- CD276, 0.5 mg/ml #5123
- E-Cadherin (CD324), 0.5 mg/ml #5085
- ICAM2, 0.5 mg/ml #5107
- Adhesion Peptides
- Collagen Hybridizing Peptides
-
Recombinant Adhesion Proteins
- Reagents
- Assays
PhotoGel®-RUT
Methacrylated Gelatin with Ruthenium Kit
Catalog #5273

PhotoGel®-RUT
Methacrylated Gelatin with Ruthenium Kit
Catalog #5273
PhotoGel®-RUT is a methacrylated gelatin kit. These 3D gelatin hydrogels can be prepared at various concentrations and photocrosslinked (400-450nm) to provide various gel stiffness. Each kit contains 1 gram (2 x 500 mg) of high DOM methacrylated gelatin and ruthenium photoinitiator.
Product Description
Advanced BioMatrix offers PhotoGel® RUT Kit, a purified methacrylated gelatin kit for visible light (400-450 nm) photocrosslinkable hydrogels. PhotoGel® provides 3D tunable gels with the unique attributes to be prepared at various concentrations and crosslinked to provide various gel stiffness.
This PhotoGel® kit consists of purified methacrylated porcine gelatin and a Visible Light Photoinitiator (400-450 nm). The gelatin is type A 300 bloom.
Table 1:
Item | Catalog Number | Package Size | Storage Temperature |
Methacrylated Gelatin | #5208 | 1 gram (2 x 500 mg) | -20°C |
Ruthenium Photoinitiator | #5246 | 100 mg | Room Temperature |
Sodium Persulfate Photoinitiator | #5247 | 500 mg | Room Temperature |
PhotoGel® is produced from methacrylated gelatin where the gelatin has been modified by reacting the free amines, primarily the ε-amines groups of the lysine residues as well as the a-amines groups on the N-termini. >75% of the total lysine residues of the gelatin molecule have been methacrylated. The photoinitiator consists of Ruthenium and Sodium Persulfate to be formulated in 1X cell culture media or PBS, which allows visible light photocrosslinking of the collagen at 400-450 nm.
For aseptic work, we recommend filtering the solubilized ruthenium and sodium persulfate through 0.2 micron button filters, as the photoinitiators do not come sterile.
Parameter, Testing, and Method | Methacrylated Gelatin #5208 |
Sterilization Method | Filtration |
Sterility - USP modified | No growth |
Form | Lyophilized Powder |
Package Size | 1 gram (2 x 500 mg) |
Storage Temperature | -20°C |
Shelf Life | Minimum of 6 months from date of receipt |
Degree of Methacrylation | > 75% |
Gelatin Source |
Type A, 300 Bloom, Porcine |
Hydrogel Young's Modulus E (Pa) |
Characteristic |
Directions for Use
Download the full PDF version or continue reading below:
3D Hydrogel Preparation:
Note: Employ aseptic practices to maintain the sterility of the product throughout the preparation and handling of the collagen and other solutions.
Note: The following instructions are for a 10% gelatin methacrylate solution. Recommended concentrations are 5-20%.
- Warm 10 mL of warm, sterile 1X PBS or 1X cell culture media to >60°C.
- Add the 10 mL’s of warmed solution to the amber vial containing 1 gram of lyophilized gelatin methacrylate.
- Mix on a shaker table or rotator plate until fully solubilized. Keep warm (>37°C) if possible (eg. place your rotator in an incubator) to help with full solubilization.
- Calculate the volume of photoinitiator to add by multiplying the volume of solubilized gelatin by 0.02. If the resulting number is 200 ul, for example, you will add 200 ul of ruthenium and 200 ul of sodium persulfate.
- Solubilize the required amount of ruthenium (per step 4) at a concentration of 37.4 mg/ml in 1X PBS or cell culture media.
- Solubilize the required amount of sodium persulfate (per step 4) at a concentration of 119 mg/ml in 1X PBS or cell culture media.
- Add the ruthenium to the gelatin solution and fully mix until solution is homogeneous.
- Add the sodium persulfate to the gelatin/ruthenium solution and mix until solution is homogeneous.
- Add your cells to the gelatin/photoinitiator solution.
- Dispense your gelatin/photoinitiator/cell solution into the desired dish (ie. 6-well plate, 48-well plate).
- For photocrosslinking, place printed structure directly under a 400-450 nm visible light crosslinking source.
Longer exposure allows more crosslinking, though each cell type withstands different degrees of light and free radicals (generated by the photoinitiator) that mediate crosslinking.
Any excess material can be refrigerated and stored. The material will gel. Warm back up to >30°C for it to become liquid again. We recommend only adding photoinitiator to the amount of gelatin to be used at that time.
Product Q & A
You can digest it with Proteinase K, though it kills the cells in the process.
Product Applications
Read our Methacrylated Extracellular Matrices eBrochure Here
PhotoGel® Gelatin methacrylate can be used to form cross-linked hydrogels for tissue engineering[1] and 3D printing. The common forms of 3D printing using Lifeink® 300 include extrusion[2][3][4], inkjet[5] and photolithography[6]).
PhotoGel® has been used for endothelial cell morphogenesis,[7] cardiomyocytes,[8] epidermal tissue[9], injectable tissue constructs[10], bone differentiation[11], and cartilage regeneration[12].
Gelatin methacrylate has been explored in drug delivery applications in the form of microspheres[13] and hydrogels[14].
References:
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2878615/
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4040163/
- https://www.ncbi.nlm.nih.gov/pubmed/24112804
- https://www.ncbi.nlm.nih.gov/pubmed/20387987
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5380738/
- https://www.ncbi.nlm.nih.gov/pubmed/28349897/
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3643201/
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551408/
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4608855/
- http://pubs.acs.org/doi/abs/10.1021/bm401533y
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4252258/
- https://www.ncbi.nlm.nih.gov/pubmed/24590160
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4293288/
- http://www.ijpsonline.com/articles/preparation-and-characterization-of-gelatinpolymethacrylic-acid-interpenetrating-polymeric-network-hydrogels-as-a-phsensitive-deli.html
Product References
References for PhotoGel®:
Rothrauff, Benjamin B., et al. "Efficacy of thermoresponsive, photocrosslinkable hydrogels derived from decellularized tendon and cartilage extracellular matrix for cartilage tissue engineering." Journal of tissue engineering and regenerative medicine 12.1 (2018): e159-e170.
Rothrauff, Benjamin B., et al. "Anatomical region-dependent enhancement of 3-dimensional chondrogenic differentiation of human mesenchymal stem cells by soluble meniscus extracellular matrix." Acta biomaterialia 49 (2017): 140-151.
Rothrauff, Benjamin B., Guang Yang, and Rocky S. Tuan. "Tissue-specific bioactivity of soluble tendon-derived and cartilage-derived extracellular matrices on adult mesenchymal stem cells." Stem cell research & therapy 8.1 (2017): 133.
Bridge, Jack Christopher, et al. "Electrospun gelatin-based scaffolds as a novel 3D platform to study the function of contractile smooth muscle cells in vitro." Biomedical Physics & Engineering Express 4.4 (2018): 045039.
Capella-Monsonís, Héctor, et al. "Scaffolds for tendon tissue engineering." Handbook of Tissue Engineering Scaffolds: Volume One. Woodhead Publishing, 2019. 259-298.
Product Certificate of Analysis
No result for .
Product Disclaimer
This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.