-
Collagen
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Type I - Atelocollagen
- PureCol® Solution, 3 mg/ml (bovine) #5005
- Nutragen® Solution, 6 mg/ml (bovine) #5010
- FibriCol® Solution, 10 mg/ml (bovine) #5133
- PureCol® EZ Gel, Solution, 5 mg/ml (bovine) #5074
- PureCol® Lyophilized, 15 mg (bovine) #5006
- VitroCol® Solution, 3 mg/ml (human) #5007
- VitroCol® Lyophilized, 15 mg (human) #5008
- Type I - Telocollagen
- Type I - Insoluble Collagen
- Type I - Bioinks
- Type II Collagen
- Type III Collagen
- Type IV Collagen
- Collagen Standard
- PureCol® Collagen Coated Plates
- Collagen Scaffolds
- Collagen Hybridizing Peptides
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Type I - Atelocollagen
- Tunable Stiffness
- CytoSoft® Rigidity Plates
- Bioprinting
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3D Hydrogels
- Thermoreversible Hydrogel
- Silk Fibroin
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Type I Collagen for 3D Hydrogels
- PureCol® Solution, 3 mg/ml (bovine) #5005
- Nutragen® Solution, 6 mg/ml (bovine) #5010
- FibriCol® Solution, 10 mg/ml (bovine) #5133
- PureCol® EZ Gel, Solution, 5 mg/ml (bovine) #5074
- VitroCol® Solution, 3 mg/ml (human) #5007
- TeloCol®-3 Solution, 3 mg/ml (bovine) #5026
- TeloCol®-6 Solution, 6 mg/ml (bovine) #5225
- TeloCol®-10 Solution, 10 mg/ml (bovine) #5226
- RatCol® for 3D gels, Solution, 4 mg/ml (rat) #5153
- HyStem® Thiolated Hyaluronic Acid
- Methacrylated Collagen
- Methacrylated Gelatin
- Methacrylated Hyaluronic Acid
- Diacrylates
- Collagen Sponges
- Extracellular Matrices
- HyStem / Hyaluronic Acid
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Adhesion Peptides / Proteins
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Recombinant Adhesion Proteins
- CD2, 0.5 mg/ml #5086
- CDH3, 0.5 mg/ml #5124
- CDH13, 0.5 mg/ml #5125
- CD14, 0.5 mg/ml #5089
- CDH18, 0.5 mg/ml #5090
- CD40, 0.5 mg/ml #5093
- CD86, 0.5 mg/ml #5096
- CD164, 0.5 mg/ml #5100
- CD270, 0.5 mg/ml #5127
- CD274, 0.5 mg/ml #5126
- CD276, 0.5 mg/ml #5123
- E-Cadherin (CD324), 0.5 mg/ml #5085
- ICAM2, 0.5 mg/ml #5107
- Adhesion Peptides
- Collagen Hybridizing Peptides
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Recombinant Adhesion Proteins
- Reagents
- Assays
PhotoPluronic®
Pluronic F-127 Diacrylate
Catalog #5342
PhotoPluronic®
Pluronic F-127 Diacrylate
Catalog #5342
PhotoPluronic® contains 2 grams of Pluronic F-127 Diacrylate. PhotoPluronic is unique in that it can gel at 37C, thermoreverse into liquid at 2C, or be photocrosslinked to form a robust hydrogel. Structures 3D bioprinted with PhotoPluronic can also be photocrosslinked to improve structural integrity.
Product Description
Advanced BioMatrix offers PhotoPluronic® - Pluronic F-127 diacrylate. PhotoPluronic® is water soluble and can be photocrosslinked in the presence of a photoinitiator (not included) to form a robust hydrogel. PhotoPluronic® is also unique in that it can transition from liquid to gel by incubating at 37C, or from gel to liquid by refrigeration at 2-8C. This process can be repeated multiple times (prior to photocrosslinking). The kit comes with 2 grams of non-sterile powder.
Item | Catalog Number | Photoinitiator Included |
PhotoPluronic | #5342-2GM | N/A |
Parameter, Testing, and Method | PhotoPluronic #5342 |
Sterilization Method | Non-sterile |
Form | Powder |
Package Size | 2 grams |
Storage Temperature | 2-8C |
Shelf Life | Minimum of 6 months from date of receipt |
Shelf Life After Reconstitution | 1 Month |
Purity by NMR | >95% |
Molecular Weight | ~12,600 |
Solubility | Water soluble |
Directions for Use
- Solubilize PhotoPluronic® by adding cold water or 1X PBS. Recommended concentrations range from 5-40%.
- Continue mixing (shaker table or rotator plate) in a cold environment (2-10C) until fully solubilized.
- Add desired photoinitiator at this step if desired. Recommended photoinitiator concentrations can be found below.
- Sterile filter through 0.45 micron button filter prior to use if sterility is desired. Keep Pluronic cold, as it will drastically increase viscosity or form a gel as it warms up.
- If there are bubbles, centrifuge the pluronic.
- At this stage, the PhotoPluronic® can be added to a syringe for 3D bioprinting, or used for the desired application.
- Note: Longer exposure allows more crosslinking, though each cell type withstands different degrees of UV light and free radical exposure (generated by the photoinitiator) that mediates crosslinking.
Photoinitiator | Photoinitiator Solubilization | Amount to add to PhotoPluronic Solution |
Irgacure | 100 mg/ml in Methanol | Multiply total volume of PhotoPluronic by 0.01. Add this amount of Irgacure solution. |
LAP | 17 mg/ml in 1X PBS or Media | Multiply total volume of PhotoPluronic by 0.02. Add this amount of LAP solution. |
Ruthenium Sodium Persulfate |
Ruthenium - 37.4 mg/ml in 1X PBS or Media Sodium Persulfate - 119 mg/ml in 1X PBS or Media |
Multiply total volume of PhotoPluronic by 0.02. Add this amount of both Ruthenium AND Sodium Persulfate solutions. |
Product Certificate of Analysis
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Product Videos
Product Disclaimer
This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.