PhotoPluronic®
Pluronic F-127 Diacrylate
Catalog #5342
PhotoPluronic®
Pluronic F-127 Diacrylate
Catalog #5342
PhotoPluronic® contains 2 grams of Pluronic F-127 Diacrylate. PhotoPluronic is unique in that it can gel at 37C, thermoreverse into liquid at 2C, or be photocrosslinked to form a robust hydrogel. Structures 3D bioprinted with PhotoPluronic can also be photocrosslinked to improve structural integrity.
Product Description
Advanced BioMatrix offers PhotoPluronic® - Pluronic F-127 diacrylate. PhotoPluronic® is water soluble and can be photocrosslinked in the presence of a photoinitiator (not included) to form a robust hydrogel. PhotoPluronic® is also unique in that it can transition from liquid to gel by incubating at 37C, or from gel to liquid by refrigeration at 2-8C. This process can be repeated multiple times (prior to photocrosslinking). The kit comes with 2 grams of non-sterile powder.
| Item | Catalog Number | Photoinitiator Included |
| PhotoPluronic | #5342-2GM | N/A |
| Parameter, Testing, and Method | PhotoPluronic #5342 |
| Sterilization Method | Non-sterile |
| Form | Powder |
| Package Size | 2 grams |
| Storage Temperature | 2-8C |
| Shelf Life | Minimum of 6 months from date of receipt |
| Shelf Life After Reconstitution | 1 Month |
| Purity by NMR | >95% |
| Molecular Weight | ~12,600 |
| Solubility | Water soluble |
Directions for Use
- Solubilize PhotoPluronic® by adding cold water or 1X PBS. Recommended concentrations range from 5-40%.
- Continue mixing (shaker table or rotator plate) in a cold environment (2-10C) until fully solubilized.
- Add desired photoinitiator at this step if desired. Recommended photoinitiator concentrations can be found below.
- Sterile filter through 0.45 micron button filter prior to use if sterility is desired. Keep Pluronic cold, as it will drastically increase viscosity or form a gel as it warms up.
- If there are bubbles, centrifuge the pluronic.
- At this stage, the PhotoPluronic® can be added to a syringe for 3D bioprinting, or used for the desired application.
- Note: Longer exposure allows more crosslinking, though each cell type withstands different degrees of UV light and free radical exposure (generated by the photoinitiator) that mediates crosslinking.
| Photoinitiator | Photoinitiator Solubilization | Amount to add to PhotoPluronic Solution |
| Irgacure | 100 mg/ml in Methanol | Multiply total volume of PhotoPluronic by 0.01. Add this amount of Irgacure solution. |
| LAP | 17 mg/ml in 1X PBS or Media | Multiply total volume of PhotoPluronic by 0.02. Add this amount of LAP solution. |
|
Ruthenium Sodium Persulfate |
Ruthenium - 37.4 mg/ml in 1X PBS or Media Sodium Persulfate - 119 mg/ml in 1X PBS or Media |
Multiply total volume of PhotoPluronic by 0.02. Add this amount of both Ruthenium AND Sodium Persulfate solutions. |
Product Certificate of Analysis
Product Videos
Product Disclaimer
This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
