Product Description

Heparan sulfate is a sulfated polysaccharide produced by all mammalian cells. It is displayed on the cell surface or secreted into the extracellular matrix where it interacts with signaling proteins, structural proteins and enzymes. Heparan sulfate biosynthesis consists of polymerization of the polysaccharide chain on a core protein followed by addition of sulfate groups. About two dozen enzymes are involved in its biosynthesis.

Heparan sulfate produced from natural sources is a heterogeneous mixture of chain lengths and sulfate patterns. Heparan sulfate composition and length varies between tissues and cell types based primarily on the expression of biosynthetic enzymes in the cell.

Heparan sulfate functions via its interaction with binding proteins. Binding is driven by the interaction of sulfate groups with basic amino acids on the protein surface. The arrangement and abundance of sulfate groups determines the affinity of the interaction with each protein having different requirements for high affinity binding. Heparan sulfate’s interaction with hundreds of proteins influences a wide range of developmental, physiological and pathophysiological processes. Heparan sulfate functions in diverse ways.

Some examples include,
• acting as a co-receptor for receptor-ligand interactions
• oligomerizing proteins
• inducing protein conformation changes
• acting as a scaffold for protein-protein interac<ons
• sequestering or protecting proteins

Because it is involved in diverse physiological systems, many researchers encounter a role for heparan sulfate in the system that they are investigating. Advanced BioMatrix provides the research community with high quality research reagents to enable their investigations.

 

Parameter, Testing, and Method Heparan Sulfate
Form White Powder
Package Size 1 mg
Source Recombinant
Storage Temperature -20°C prior to reconstitution and 2-10°C after reconstitution 
Shelf Life Minimum of 6 months from date of receipt
DNA Content - A260 <1%
Protein Content - BCA Assay <1%
Sterility Non Sterile
Sulfate Content

rHS01 (#5312) - 53 sulfate groups per 100 disaccharides

rHS02 (#5313) - 64 sulfate groups per 100 disaccharides

rHS09 (#5334) - 245 sulfate groups per 100 disaccharides

rHS29 (#5335) - 205 sulfate groups per 100 disaccharides

Sterility

This product is non-sterile

Directions for Use

Our Recombinant Heparan Sulfate can be reconstituted at a concentration up to 1 mg/ml in sterile water or your desired buffer solution. 

This product is non-sterile.

Heparan Binding Assay

Product Q & A

It is Heparan Sulfate only. The protein has been removed by beta-elimination. 

Product Certificate of Analysis

No result for .

Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.