Product Description

Type V Collagen provides structure and strength to connective tissue and is found in many places in the body, especially skin, lung, intestinal walls and walls of blood vessels. This Type V Collagen product is isolated from placenta and is purified using a multi-step process with a final purity of >95%.  Type V collagen forms a triple helix composed of a Alpha 1(V)2 Alpha 2(V) heterotrimer.

The product is supplied as a sterile solution at 0.5 mg per vial at approximately 0.15 to 0.30 mg/ml in 100 mM acetic acid. A Certificate of Analysis is available with the purchase of each product. Type V collagen product is provided in a user-friendly packaging for use and storage.

Type V Collagen is typically used as a thin coating on tissue culture surfaces.  Specific instructions are found in the Directions for Use. This product is generally used in vitro as a substrate scaffold to enhance cell attachment, adherence and proliferation. Type V collagen may be used to culture a variety of cell types.

 

Parameter, Testing, and Method Type V Collagen #5168
Sterilization Method Filtration
Form Solution
Package Size 0.5 mg (~2 mL)
Storage Temperature 2-10°C
Shelf Life Minimum of 6 months from date of receipt

Collagen Concentration - Biuret

0.15-0.3 mg/mL
pH 2.0-3.5

Electrophoretic Pattern - Coomassie Blue

Characteristic
Endotoxin - LAL <5.0 EU/mL
Cell Attachment Assay Pass
Source Bovine Placenta

Directions for Use

Download the full PDF version or continue reading below:

Coating Procedure

Note: Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.

  1. Remove required quantity of collagen from the bottle and dispense into a dilution vessel.
  2. Dilute Type V Collagen with 100 mM acetic acid to ~10 µg/ml.
  3. Swirl contents gently until material is completely mixed.
  4. Add appropriate amount of diluted Type V Collagen material to the culture surface ensuring that the entire surface is coated.
  5. Incubate at room temperature, covered, for 1-2 hours.
  6. After incubation, aspirate any remaining material.
  7. Rinse coated surfaces carefully with sterile medium or PBS, avoid scratching surfaces.
  8. Coated surfaces are ready for use. They may also be stored at 2-8°C damp or air dried if sterility is maintained.

Product Certificate of Analysis

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Safety and Documentation

Safety Data Sheet

Certificate of Origin

Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.