Product Description

Advanced BioMatrix offers PhotoGel®-INK, a sterile, pre-formulated 10% methacrylated gelatin ink with 0.25% LAP for rapid 3D bioprinting with the BIONOVA X. 

The PhotoGel® consists of purified methacrylated porcine gelatin (25 mL) and LAP photoinitiator. The gelatin is type A 300 bloom.  

PhotoGel® is produced from methacrylated gelatin where the gelatin has been modified by reacting the free amines, primarily the ε-amines groups of the lysine residues as well as the a-amines groups on the N-termini. ~50% of the total lysine residues of the gelatin molecule have been methacrylated. 

Parameter, Testing, and Method Methacrylated Gelatin 
Sterilization Method Filtration
Sterility - USP modified No growth
Form Sterile Solution
Package Size 25 mL
Storage Temperature 2-8°C
Shelf Life Minimum of 6 months from date of receipt
Degree of Methacrylation ~50%

Source

Type A, 300 Bloom, Porcine Gelatin

Hydrogel Young's Modulus E (Pa)

Characteristic

Product Q & A

You can digest it with Proteinase K, though it kills the cells in the process. 

Product Applications

Read our Methacrylated Extracellular Matrices eBrochure Here

PhotoGel® Gelatin methacrylate can be used to form cross-linked hydrogels for tissue engineering[1] and 3D printing. The common forms of 3D printing using Lifeink® 300 include extrusion[2][3][4], inkjet[5] and photolithography[6]).

PhotoGel® has been used for endothelial cell morphogenesis,[7] cardiomyocytes,[8] epidermal tissue[9], injectable tissue constructs[10], bone differentiation[11], and cartilage regeneration[12].

Gelatin methacrylate has been explored in drug delivery applications in the form of microspheres[13] and hydrogels[14].

References:

  1. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2878615/
  2. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4040163/
  3. https://www.ncbi.nlm.nih.gov/pubmed/24112804
  4. https://www.ncbi.nlm.nih.gov/pubmed/20387987
  5. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5380738/
  6. https://www.ncbi.nlm.nih.gov/pubmed/28349897/
  7. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3643201/
  8. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551408/
  9. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4608855/

Product References

References for PhotoGel®:

 

Rothrauff, Benjamin B., et al. "Efficacy of thermoresponsive, photocrosslinkable hydrogels derived from decellularized tendon and cartilage extracellular matrix for cartilage tissue engineering." Journal of tissue engineering and regenerative medicine 12.1 (2018): e159-e170.

Rothrauff, Benjamin B., et al. "Anatomical region-dependent enhancement of 3-dimensional chondrogenic differentiation of human mesenchymal stem cells by soluble meniscus extracellular matrix." Acta biomaterialia 49 (2017): 140-151.

Rothrauff, Benjamin B., Guang Yang, and Rocky S. Tuan. "Tissue-specific bioactivity of soluble tendon-derived and cartilage-derived extracellular matrices on adult mesenchymal stem cells." Stem cell research & therapy 8.1 (2017): 133.

Bridge, Jack Christopher, et al. "Electrospun gelatin-based scaffolds as a novel 3D platform to study the function of contractile smooth muscle cells in vitro." Biomedical Physics & Engineering Express 4.4 (2018): 045039.

Capella-Monsonís, Héctor, et al. "Scaffolds for tendon tissue engineering." Handbook of Tissue Engineering Scaffolds: Volume One. Woodhead Publishing, 2019. 259-298.

Product Certificate of Analysis

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Safety and Documentation

 SDS

Certificate of Origin

Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.