Product Description

Poly-D-Lysine is a synthetic amino acid chain that is positively charged and widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces.

The molecular weight of Poly-D-Lysine can vary significantly with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (>300,000 Da) having more binding sites per molecule.  This product’s molecular weight ranges from 70,000 to 150,000 Da.

Poly-D-Lysine is supplied in a 50 ml amber bottle containing 5 mg of Poly-D-Lysine. The product has been sterilized and is ready-to-use after proper addition of cell culture water.

Parameter, Testing, and Method Poly-D-Lysine #5174
Form Powder
Package Size 5 mg
Storage Temperature -20°C
Sterilization Method Irradiation
Molecular Weight 70,000 - 150,000 Da
Sterility - USP modified No Growth
Cell Attachment Assay Pass
Source Synthetic
Shelf Life Minimum of 6 months from date of receipt

Directions for Use

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Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.  To maintain sterility, perform all operations in a laminar flow hood.

  1. A typical working concentration is 0.1 mg/ml. To achieve a 0.1 mg/ml concentration, add 50 ml of tissue culture grade water or PBS to the 50 ml bottle. If a different concentration is desired, transfer desired volume of solution from the bottle to a dilution vessel. Dilute to desired concentration using tissue culture grade water or PBS.
  2. Add appropriate amount of diluted material to culture surface. Typically, 1 ml per 25 cm2is used. Rock gently to ensure uniform coating of culture surface.

Option A:

Allow to incubate for 60 minutes at room temperature, remove excess solution by aspiration.

  1. Thoroughly rinse surface with tissue culture grade water and aspirate rinse water.
  2. Incubate and allow to dry at room temperature or 37°C, covered, for at least 2 hours. Coated cultureware can be stored for up to 1 week. Go to Step 9

Option B:

  1. Allow to incubate overnight at room temperature, remove excess solution by aspiration.
  2. Thoroughly rinse surface with tissue culture grade water and aspirate rinse water.
  3. Incubate and allow to dry at room temperature or 37°C, covered, for at least 2 hours. Coated cultureware can be stored for up to 1 week.
  4. Introduce medium and cells to the culture surface.

Store remaining Poly-D-Lysine solution at -10 to 30°C

Product References

References for Poly-D-Lysine:

Albuquerque, Cristóvão, et al. "Preparation of coverslips for neuronal cultures." Cold Spring Harbor Protocols 2009.8 (2009): pdb-prot5272.

Unsain, Nicolas, et al. "Production and isolation of axons from sensory neurons for biochemical analysis using porous filters." JoVE (Journal of Visualized Experiments) 89 (2014): e51795.

Li, Ling, Mizuho Fukunaga-Kalabis, and Meenhard Herlyn. "Isolation, characterization, and differentiation of human multipotent dermal stem cells." Skin Stem Cells. Humana Press, Totowa, NJ, 2013. 235-246.

Baik, Matthew, et al. "Identification of invadopodia by TKS5 staining in human cancer lines and patient tumor samples." MethodsX 6 (2019): 718-726.

Tammia, Markus, et al. "Egr2 overexpression in Schwann cells increases myelination frequency in vitro." Heliyon 4.11 (2018): e00982.

Boularaoui, Selwa Mokhtar, et al. "Efficient transdifferentiation of human dermal fibroblasts into skeletal muscle." Journal of tissue engineering and regenerative medicine 12.2 (2018): e918-e936.

Johnstone, Aaron D., et al. "Developmental axon degeneration requires TRPV1-dependent Ca2+ influx." eNeuro 6.1 (2019).

Product Certificate of Analysis

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Safety and Documentation

Safety Data Sheet

Certificate of Origin

Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.