Product Description

Advanced BioMatrix offers PhotoGel® , a purified and lyophilized methacrylated gelatin for photocrosslinkable hydrogels. PhotoGel® provides 3D tunable gels with the unique attributes to be prepared at various concentrations and crosslinked to provide various gel stiffness.

The PhotoGel® kit consists of purified methacrylated porcine gelatin and a UV Photoinitiator (365 nm). The gelatin is type A 300 bloom. This individual product only contains the methacrylated gelatin. Please visit a PhotoGel® Kit page for purchasing the entire photocrosslinking kit.

Item Catalog Number Package Size Storage Temperature
Methacrylated Gelatin #5208 1 gram (2 x 500 mg) -20°C

 

PhotoGel® is produced from methacrylated gelatin where the gelatin has been modified by reacting the free amines, primarily the ε-amines groups of the lysine residues as well as the a-amines groups on the N-termini. >75% of the total lysine residues of the gelatin molecule have been methacrylated. The photoinitiator consists of Irgacure 2959 to be formulated in methanol (methanol not included) which allows UV crosslinking of the collagen at 365 nm.

Parameter, Testing, and Method Methacrylated Gelatin #5208
Sterilization Method Filtration
Sterility - USP modified No growth
Form Lyophilized Powder
Package Size 1 gram (2 x 500 mg)
Storage Temperature -20°C
Shelf Life Minimum of 6 months from date of receipt
Degree of Methacrylation > 75%

Source

Type A, 300 Bloom, Porcine Gelatin

Hydrogel Young's Modulus E (Pa)

Characteristic

Product Applications

PhotoGel® Gelatin methacrylate can be used to form cross-linked hydrogels for tissue engineering[1] and 3D printing. The common forms of 3D printing using Lifeink® 300 include extrusion[2][3][4], inkjet[5] and photolithography[6]).

PhotoGel® has been used for endothelial cell morphogenesis,[7] cardiomyocytes,[8] epidermal tissue[9], injectable tissue constructs[10], bone differentiation[11], and cartilage regeneration[12].

Gelatin methacrylate has been explored in drug delivery applications in the form of microspheres[13] and hydrogels[14].

References:

  1. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2878615/
  2. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4040163/
  3. https://www.ncbi.nlm.nih.gov/pubmed/24112804
  4. https://www.ncbi.nlm.nih.gov/pubmed/20387987
  5. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5380738/
  6. https://www.ncbi.nlm.nih.gov/pubmed/28349897/
  7. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3643201/
  8. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551408/
  9. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4608855/

Product References

References for PhotoGel®:

 

Rothrauff, Benjamin B., et al. "Efficacy of thermoresponsive, photocrosslinkable hydrogels derived from decellularized tendon and cartilage extracellular matrix for cartilage tissue engineering." Journal of tissue engineering and regenerative medicine 12.1 (2018): e159-e170.

Rothrauff, Benjamin B., et al. "Anatomical region-dependent enhancement of 3-dimensional chondrogenic differentiation of human mesenchymal stem cells by soluble meniscus extracellular matrix." Acta biomaterialia 49 (2017): 140-151.

Rothrauff, Benjamin B., Guang Yang, and Rocky S. Tuan. "Tissue-specific bioactivity of soluble tendon-derived and cartilage-derived extracellular matrices on adult mesenchymal stem cells." Stem cell research & therapy 8.1 (2017): 133.

Bridge, Jack Christopher, et al. "Electrospun gelatin-based scaffolds as a novel 3D platform to study the function of contractile smooth muscle cells in vitro." Biomedical Physics & Engineering Express 4.4 (2018): 045039.

Capella-Monsonís, Héctor, et al. "Scaffolds for tendon tissue engineering." Handbook of Tissue Engineering Scaffolds: Volume One. Woodhead Publishing, 2019. 259-298.

Product Certificate of Analysis

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Safety and Documentation

Safety Data Sheet

Certificate of Origin

Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.