Product Description

Collagen Hybridizing Peptides (CHP) conjugates from Advanced BioMatrix are labeled with 5-FAM (F-CHP, Catalog No. 5264-60UG) / sulfo-Cyanine3 (R-CHP, Catalog No. 5276-60UG) for fluorescence detection, or biotin (B-CHP, Catalog No. 5265-UG) for avidin/streptavidin-mediated detection.

The triple helix is the hallmark protein structure of collagen. During tissue remodeling, the triple helical collagen molecules are degraded by specific proteases (e.g., MMP or cathepsin K) and become unfolded at body temperature. The Collagen Hybridizing Peptide (CHP) is a synthetic peptide that can specifically bind to such denatured collagen strands through hydrogen bonding, both in histology, in vivo, and in vitro (3D cell culture). By sharing the structural motif and the Gly-X-Y repeating sequence of natural collagen, CHP has a strong capability to hybridize with denatured collagen strands, in a fashion that is similar to a DNA fragment annealing to its complimentary DNA strand during PCR. CHP is an extremely specific probe for unfolded collagen molecules: it has negligible affinity to intact collagen molecules due to the lack of binding sites; it is also inert towards non-specific binding because of its neutral and hydrophilic nature.

Parameter, Testing, and Method CHP Cy3 #5276
Specialty Straight-forward fluore-scence detection in red
Formula C144H198N33O46S2
Molecular Weight 3191.44 g/mol
Ex/Em 548 nm / 563 nm
Synonym Collagen Mimetic Peptide, CMP
Solubility Aqueous Buffers
Storage -20°C as powder for long term storage. 4°C after reconstitution in water. Protect from light.
Source Synthetic
Shelf Life Minimum of 6 months from date of receipt

Storage/Stability:

The product ships on ambient. Store the product at -20°C.

Features of CHP reagents

  • More informative, reliable and convenient than zymography, DQ collagen, SHG, and TEM
  • High affinity and unparalleled specificity to collagen with essentially no nonspecific binding
  • Applicable to denatured collagens of all subtypes and from all species; binding relying on collagen’s secondary structure instead of specific epitopes
  • Suitable for both frozen and paraffin-embedded sections with no need for antigen retrieval
  • A non-antibody approach with no species restrictions, compatible for co-staining with any antibody
  • Small size (2% of IgG by MW) enabling facile tissue penetration during whole tissue staining (with no need for sectioning)
  • Stable in solution under 4 °C, eliminating the need to aliquot for storage

Product Q & A

The CHP is fairly stable. Therefore, it is possible to reuse a dilute solution that has been heated already, although the intensity of the stain may decrease and results may not be as optimal. To reuse the dilute solution, it needs to be heated to 80C and cooled down again immediately prior to reuse.

Product References

References for Collagen Hybridizing Peptides:

1. Targeting and mimicking collagens via triple helical peptide assemblies. Current Opinion in Chemical Biology, 2013, 17, 968–975.

2. In situ imaging of tissue remodeling with collagen hybridizing peptides. ACS Nano, 2017, 11, 9825–9835.

3. Targeting collagen strands by photo-triggered triple-helix hybridization. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109, 14767–14772.

4. Visualizing collagen proteolysis by peptide hybridization: From 3D cell culture to in vivo imaging. Biomaterials, 2018, 183, 67–76. 

5. Direct detection of collagenous proteins by fluorescently labeled collagen mimetic peptides. Bioconjugate Chemistry, 2013, 24, 9–16.

6. Molecular level detection and localization of mechanical damage in collagen enabled by collagen hybridizing peptides. Nature Communications, 2017, 8, 14913, doi: 10.1038/ncomms14913.

7. Molecular assessment of collagen denaturation in decellularized tissues using a collagen hybridizing peptide. Acta Biomaterialia, 2017, 53, 268–278.

Product Certificate of Analysis

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Safety and Documentation

Safety Data Sheet

Certificate of Origin

Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.