Product Description

Poly-L-Ornithine is a synthetic amino acid chain that is positively charged having one hydrobromide per unit of ornithine.  Poly-L-Ornithine is widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces.

The molecular weight of Poly-L-Ornithine can vary significantly with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (>300,000 Da) having more binding sites per molecule. This product’s molecular weight ranges from 70,000 to 150,000 Da yielding a solution viscosity for easy handling while providing sufficient binding sites for cell attachment.

Poly-L-Ornithine surface coatings are designed to improve cell attachment, growth and differentiation of many cell types.   

Coated surfaces will often improve cell attachment in reduced or serum-free conditions.  This product is supplied in a sterile 50 ml package size at a concentration of 0.1 mg/ml.

Parameter, Testing, and Method Poly-L-Ornithine #5058
Form Solution
Package Size 50 mL
Storage Temperature 2-10°C
Sterilization Method Filtration
Molecular Weight 70,000 - 150,000 Da
Sterility - USP modified No Growth
Cell Attachment Assay Pass
Source Synthetic
Shelf Life Minimum of 6 months from date of receipt
Concentration 0.1 mg/mL

Directions for Use

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Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.  To maintain sterility, perform all operations in a laminar flow hood.

  1. A typical working concentration is 0.1 mg/ml. If a different concentration is desired, transfer desired volume of solution from the bottle to a dilution vessel.  Dilute to desired concentration using tissue culture grade water or PBS.
  2. Add appropriate amount of diluted material to culture surface. Typically, 1 mL per 25 cm2 is used. Rock gently to ensure uniform coating of culture surface.
  3. After 5 minutes, remove excess solution by aspiration.
  4. Thoroughly rinse surface with tissue culture grade water.
  5. Incubate and allow to dry at room temperature or 37°C, covered, for at least 2 hours.
  6. Introduce medium and cells to the culture surface.
  7. Store remaining Poly-L-Ornithine at 2 to 10°C.

Product References

References for Poly-L-Ornithine:

Chen, Eric YT, et al. "Activated charcoal composite biomaterial promotes human embryonic stem cell differentiation toward neuronal lineage." Journal of Biomedical Materials Research Part A 100.8 (2012): 2006-2017.

Boularaoui, Selwa Mokhtar, et al. "Efficient transdifferentiation of human dermal fibroblasts into skeletal muscle." Journal of tissue engineering and regenerative medicine 12.2 (2018): e918-e936.

Ergin, Ekin, et al. "Time-resolved fluorescence resonance energy transfer [TR-FRET] assays for biochemical processes." Current pharmaceutical biotechnology 17.14 (2016): 1222-1230.

Product Certificate of Analysis

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Safety and Documentation

Safety Data Sheet

Certificate of Origin

Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.