Product Description

Bovine Collagen Type II is one of a family of proteins found particularly in the flesh and connective tissues of mammals (approximately one-third of the body’s total protein). Over two dozen types of collagen have been described; Type II is found predominantly in hyaline cartilage (50% of the protein in hyaline is collagen type II) and is also found in the vitreous humor of the eye.

The product is supplied as a sterile solution at approximately 0.5 mg per vial at approximately ~.50 mg/ml in 500 mM acetic acid. Type II collagen is provided in user-friendly packaging for use and storage. Type II collagen may be used to culture a variety of cell types. Type II collagen is typically used as a coating material for cell culture studies, for the formation of 3D collagen gels, or as an antigen for antibody production.


Parameter, Testing, and Method Type II Collagen #5196
Sterilization Method Filtration
Form Solution
Package Size 0.5 mg (1 mL)
Storage Temperature 2-10°C

Collagen Concentration - Biuret

~0.5 mg/mL

Shelf Life

Minimum of 6 months from date of receipt
pH ~2.5

Electrophoretic Pattern - Coomassie Blue

Endotoxin (LAL) <5.0 EU/mL
Cell Attachment Assay Pass
Source Bovine Cartilage

Directions for Use

Recommended Volumes for 2D Coatings or 3D Hydrogels

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Coating Procedure

Note: Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.

  1. Remove required quantity of collagen from the bottle and dispense into a dilution vessel.
  2. Dilute the product with a 500 mM acetic acid solution to desired concentration.
  3. Swirl contents gently until thoroughly mixed.
  4. Add approximate amount of diluted Type II Collagen to the culture surface ensuring the entire surface is coated.
  5. Incubate at room temperature, covered for 1-2 hours.
  6. After incubation, aspirate any excess remaining material.
  7. Rinse coated surface carefully with a sterile medium or balanced salt solution. Avoid scratching surfaces.
  8. Aspirate remaining material from coated surface.
  9. Coated culture vessels are now ready to use.  The coated culture vessels may be stored at 2 to 10°C

Product Certificate of Analysis

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Safety and Documentation

Safety Data Sheet

Certificate of Origin

Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.