Product Description

Advanced BioMatrix offers PhotoDextran®, a methacrylated dextran for photocrosslinkable hydrogels or incorporating within photocrosslinkable hydrogels (such as PhotoGel, PhotoCol or PhotoHA). These hydrogels provide native-like 3D HA gels with the unique attributes to be prepared at various concentrations and photocrosslinked to provide various gel stiffness. PhotoDextran® can be ordered by itself (1 gram lyophilized sterile powder), or as a kit that comes with a photoinitiator for photocrosslinking (see table below):

Item Catalog Number Photoinitiator Included
PhotoDextran #5311 N/A
PhotoDextran with Irgacure #5331 Irgacure (365nm)
PhotoDextran with LAP #5332 LAP (405nm)
PhotoDextran with Ruthenium #5333 Ruthenium (400-450nm)

 

 

Once solubilized, the PhotoDextran® can be stored at 2-10°C for 1 month.

Parameter, Testing, and Method Methacrylated Dextran #5311
Sterilization Method Filtration
Sterility - USP modified No growth
Form Lyophilized Powder
Package Size 1 gram
Storage Temperature -20°C
Shelf Life Minimum of 6 months from date of receipt
Shelf Life After Reconstitution 1 Month
Degree of Methacrylation 45-65%
Molecular Weight 35,000-50,000 kDa
Endotoxin - IU/g <10

 

Directions for Use

3D Hydrogel Preparation:

Note: Employ aseptic practices to maintain the sterility of the product throughout the preparation.

Recommended concentrations are 50-200 mg/ml
(5-20%).

Note: The following instructions are for a 5% hydrogel. 

  1. Add 20 ml of 1X cold phosphate buffer saline (PBS) or cell culture media to the 1 gram of lyophilized methacrylated dextran powder.
  2. Mix on a shaker table or rotator plate until fully solubilized (~30 to 60 minutes) at 2-10°C.Note: Solubilization times may vary depending on the desired concentration and volume of PBS or medium added,
  3. Solubilize the photoinitiator at the recommended concentration, and add desired volume to your PhotoDextran®. See table below for concentration and volume recommendations. 
  4. Add your cells to the solution if desired. Cells can also be added on top of crosslinked hydrogels. 
  5. Dispense your PhotoDextran/photoinitiator/cell solution into the desired cultureware (i.e. 6-well plate, 48-well plate, etc...).
  6. Photocrosslink until a hydrogel of the desired stiffness has formed.
  7. Note:  Longer exposure allows more crosslinking, though each cell type withstands different degrees of UV light and free radical exposure (generated by the photoinitiator) that mediates crosslinking.
Photoinitiator Photoinitiator Solubilization  Amount to add to PhotoDextran Solution
Irgacure 100 mg/ml in Methanol Multiply total volume of PhotoDextran by 0.01. Add this amount of Irgacure solution. 
LAP 17 mg/ml in 1X PBS or Media Multiply total volume of PhotoDextran by 0.02. Add this amount of LAP solution.

Ruthenium

Sodium Persulfate

Ruthenium - 37.4 mg/ml in 1X PBS or Media

Sodium Persulfate - 119 mg/ml in 1X PBS or Media

Multiply total volume of PhotoDextran by 0.02. Add this amount of both Ruthenium AND Sodium Persulfate solutions.

Product Applications

The following graph shows the shear modulus G' (Pa) of PhotoDextran® at a 5% solution. The solution was prepared following the directions for use. Irgacure was used as the photoinitiator, and photocrosslinking was performed using 365nm light. The graph shows the hydrogel strength over time throughout continuous photocrosslinking of the solution. 

Product Certificate of Analysis

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Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.