Product Description

Polyethylene Glycol Diacrylate (PEGDA) hydrogels are powerful tools for uncovering basic cellular biology because they are considered biologically inert (“blank slate”) and their mechanical properties can be varied over a large range of moduli.

PEGDA is an emerging scaffold for tissue engineering and regenerative medicine since polymerization can occur rapidly at room temperature and requires low energy input, has high water content, is elastic, and can be customized to include a variety of biological molecules.

PEGDA is a crosslinker repeating polymer crosslinker and is >80% acrylated. It can be used as a thiol-reactive crosslinker in our HyStem systems at low concentration, or can be used to make a PEGDA-only hydrogel in the presence of a free-radical chain photoinitiator and light source. PEG-only gels do not typically support cell attachment without the incorporation of cellular attachment sites.

Applications
Regular and 3-D cell culture
Tissue engineering
Photolithography

Store PEGDA in the original vial unopened at -20°C for up to one year. Reconstituted PEGDA solutions can be stored at -20°C for ~ one month.

 

Directions for Use

Download the full Directions for Use PDF here or continue reading below:

PEGDA is prepared by dissolving the lyophilized solid in the DG Water. PEGDA hydrogels (1 mL) should be prepared in the following manner:

  1. Allow the PEGDA to come to room temperature.
  2. Under aseptic conditions add 1.0 mL of DG Water to 100 mg of PEGDA vial for a 10% (w/v) solution.
  3. Invert several times to dissolve. The solution will be clear and slightly viscous.
  4. To sterilize, filter through a 0.2 micron filter.
  5. Add desired volume of photoinitiator to PEGDA. Place the vial on a rocker or shaker and allow solids to completely dissolve (approximately 10-20 minutes).
  6. Keep solution protected from light until ready to crosslink. Pipette solution into desired format (i.e. 96 well plate) and photocrosslink.
  7. Hydrogel properties may vary depending on time of exposure and type of light. Ensure the Photocrosslinking light is in close proximity to the hydrogel solution.
  8. Freeze unused PEGDA solution at -20 °C and protected from light.

Product Applications

Read our HyStem eBrochure Here

or

Click on the title of the desired protocol to learn more: 

2D Cell Growth on HyStem Hydrogels

HyStem 3D Cell Encapsulation for Cell Delivery Applications Guide

HyStem 3D Cell Encapsulation in hydrogels using 96-well plates

HyStem 3D Cell Encapsulation in hydrogels using TC Inserts

Enzyme Digestion of HyStem Hydrogels for Recovery of Encapsulated Cells

Fluorescent Labeling of HyStem Hydrogels

Cell Recovery from Surface of HyStem Hydrogels

HyStem ECM Incorporation

HyStem Gelation Time Variation

HyStem Stiffness Variation Protocol for 7.5 mL kit

HyStem Stiffness Variation Protocol for 12.5 mL kit

Product References

Salinas CN and Anseth KS, The enhancement of chondrogenic differentiation of human mesenchymal stem cells by enzymatically regulated RGD functionalities. Biomaterials. (2008) 29:2370-7.

Kloxin AM, et al.Photodegradable Hydrogels for Dynamic Tuning of Physical and Chemical Properties Science (2009) 324, 59.

DeForest CA, Polizzotti BD, and Anseth KS, Sequential click reactions for synthesizing and patterning three-dimensional cell microenvironments Nat. Mater. (2009) 8: pp. 659-664.

Baird IS, Yau AY, and Mann BK, Mammalian cell-seeded hydrogel microarrays printed via dip-pin technology BioTech. (2008) 44:249-256.

Baek TJ et al, Photolithographic Fabrication of Poly(Ethylene Glycol) Microstructures for Hydrogel-based Microreactors and Spatially Addressed Microarrays J. Microbiol. Biotechnol. (2007) 17: 1826-1832.

Taite LJ, Rowland ML, Ruffino KA, Smith BR, Lawrence MB, West JL. Bioactive hydrogel substrates: probing leukocyte receptor-ligand interactions in parallel plate flow chamber studies. Ann Biomed Eng. (2006) 34:1705-11.

Du Y, Lo E, Ali S, Khademhosseini A. Directed assembly of cell-laden microgels for fabrication of 3D tissue constructs. Proc Natl Acad Sci U S A. (2008) 105:9522-7.

Khademhosseini A, Yeh J, Jon S, Eng G, Suh KY, Burdick JA, Langer R. Molded polyethylene glycol microstructures for capturing cells within microfluidic channels. Lab Chip. (2004) 4:425-30.

Liao H, Munoz-Pinto D, Qu X, Hou Y, Grunlan MA, Hahn MS. Influence of hydrogel mechanical properties and mesh size on vocal fold fibroblast extracellular matrix production and phenotype. Acta Biomater. (2008) 4:1161-71.

Patel PN, Smith CK, Patrick CW Jr. Rheological and recovery properties of poly(ethylene glycol) diacrylate hydrogels and human adipose tissue.J Biomed Mater Res A. (2005) 73:313-9.

Panda P et al, Stop-Flow Lithography to Generate Cell-Laden Microgel Particles. Lab Chip (2008) 8:1056-1061.

Ma PX and Elisseeff J editors, Scaffolds in Tissue Engineering, CRC Press Boca Raton, FL 2006.

Product Certificate of Analysis

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Safety and Documentation

Certificate of Origin

Safety Data Sheet

Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.