Product Description

Human CD14 (monocyte differentiation antigen CD14) is a 375 amino acid, phospholipid anchored cell surface protein. This protein is preferentially expressed on monocytes / macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide.

Full-length extracellular domain of human CD14 gene (20-345 aa) was constructed with 29 N-terminal T7/His tag and expressed in E. coli as inclusion bodies. 

Parameter, Testing, and Method CD14 #5089
Quantity 0.1 mg
Volume 0.2 mL
Concentration 0.5 mg/mL
Purity - SDS PAGE Electrophoresis > 90%
Formulation Formulated in 20 mM pH 8.0 TRIS-HCL Buffer, with proprietary formulation of NaCl, KCl, EDTA, L-Arginine, DTT and Glycerol
Form Solution
Production Type Recombinant - E. Coli
Storage Temperature -20°C
Shelf Life Minimum of 6 months from date of receipt
Sterilization Method Filtration
Cell Assay Pass
Sterility - USP modified No Growth
Accession Number NP_000582
Recombinant Protein Sequence MASMTGGQQMGRGHHHHHHGNLYFQGTTPEPCELDDEDFRC
VCNFSEPQPDWSEAFQCVSAVEVEIHAGGLNLEPFLKRVDADA
DPRQYADTVKALRVRRLTVGAAQVPAQLLVGALRVLAYSRLKEL
TLEDLKITGTMPPLPLEATGLALSSLRLRNVSWATGRSWLAELQQ
WLKPGLKVLSIAQAHSPAFSCEQVRAFPALTSLDLSDNPGLGERG
LMAALCPHKFPAIQNLALRNTGMETPTGVCAALAAAGVQPHSLDL
SHNSLRATVNPSAPRCMWSSALNSLNLSFAGLEQVPKGLPAKLR
VLDLSCNRLNRAPQPDELPEVDNLTLDGNPFLVPGTALPHEGSMN

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Poduct was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

Directions for Use

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Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.

  1. Thaw CD14 and dilute to desired concentration using serum-free medium or PBS. The final solution should be sufficiently dilute so that the volume added covers the surface evenly.
  2. Add appropriate amount of diluted material to culture surface.
  3. Incubate at room temperature for approximately 1 – 2 hours.
  4. Aspirate remaining material.
  5. Rinse plates carefully with dH2O– avoid scratching bottom surface of plates.
  6. Plates are ready for use. They may also be stored at 2-8°C damp or air dried if sterility is maintained.

Coating this recombinant protein at 1-10 ug / well (6 well plate) in T cell specific medium can be used for 1) human T cell / receptor interaction study in vitro or 2) as a breast cancer biomarker for diagnosis application when combined with CD16 antigen.

Product Certificate of Analysis

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Safety and Documentation

Safety Data Sheet

Certificate of Origin

Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.