Product Description

Advanced BioMatrix offers PhotoGel®  50% degree of methacrylation, a purified and lyophilized methacrylated gelatin (GelMA) for photocrosslinkable hydrogels. PhotoGel® provides 3D tunable gels with the unique attributes to be prepared at various concentrations and crosslinked to provide various gel stiffness.

The PhotoGel® kit consists of purified methacrylated porcine gelatin and a photoinitiator. The gelatin is type A 300 bloom. The individual product only contains the methacrylated gelatin. Please order a PhotoGel® Kit for purchasing the photocrosslinking kit.

Item Catalog Number Package Size Storage Temperature
Methacrylated Gelatin #VL3500000502 1 gram (2 x 500 mg) -20°C

 

PhotoGel® is produced from methacrylated gelatin where the gelatin has been modified by reacting the free amines, primarily the ε-amines groups of the lysine residues as well as the a-amines groups on the N-termini. >75% of the total lysine residues of the gelatin molecule have been methacrylated. The photoinitiator consists of Irgacure 2959 to be formulated in methanol (methanol not included) which allows UV crosslinking of the collagen at 365 nm.

Parameter, Testing, and Method Methacrylated Gelatin #5208
Sterilization Method Filtration
Sterility - USP modified No growth
Form Lyophilized Powder
Package Size 1 gram (2 x 500 mg)
Storage Temperature -20°C
Shelf Life Minimum of 6 months from date of receipt
Degree of Methacrylation 40-60%

Source

Type A, 300 Bloom, Porcine Gelatin

Hydrogel Young's Modulus E (Pa)

Characteristic

Directions for Use

Download the full Directions for Use PDF

 NOTE: For LAP photoinitiator, the basic recommendations will result in a ~0.03% final LAP concentration. For some applications, such as DLP printing, the final LAP concentration should be increased to ~0.25-0.5% to accelerate and improve crosslinking. 

Product Q & A

You can digest it with Proteinase K, though it kills the cells in the process. 

Product Applications

Read our Methacrylated Extracellular Matrices eBrochure Here

PhotoGel® Gelatin methacrylate can be used to form cross-linked hydrogels for tissue engineering[1] and 3D printing. The common forms of 3D printing using Lifeink® 300 include extrusion[2][3][4], inkjet[5] and photolithography[6]).

PhotoGel® has been used for endothelial cell morphogenesis,[7] cardiomyocytes,[8] epidermal tissue[9], injectable tissue constructs[10], bone differentiation[11], and cartilage regeneration[12].

Gelatin methacrylate has been explored in drug delivery applications in the form of microspheres[13] and hydrogels[14].

References:

  1. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2878615/
  2. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4040163/
  3. https://www.ncbi.nlm.nih.gov/pubmed/24112804
  4. https://www.ncbi.nlm.nih.gov/pubmed/20387987
  5. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5380738/
  6. https://www.ncbi.nlm.nih.gov/pubmed/28349897/
  7. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3643201/
  8. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551408/
  9. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4608855/

Product Certificate of Analysis

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Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.