Product Description

LifeSupport® is the core component and industry standard for FRESH bioprinting.

FRESH stands for Freeform Reversible Embedding of Suspended Hydrogels.

Each LifeSupport® printing kit comes with 5 units of 2 grams of sterile LifeSupport® powder. One gram of powder hydrates to approximately 10 mL of FRESH support material.

Read the groundbreaking publication using LifeSupport® here: https://science.sciencemag.org/content/365/6452/482

Parameter, Testing, and Method LifeSupport® #5244
Sterilization Method Irradiation
Sterility - USP modified No growth
Form Grounded Powder
Bioprinting Supports Lifeink® Collagen Bioprinting
Average Particle Size Diameter 15-50 microns
Package Size 10 grams of powder (5 x 2 grams)
Rheology Bingham Plastic Properties
Shelf Life Minimum of 6 months from date of receipt
Source Material Bovine Gelatin

Directions for Use

Download the entire DFU here.

Product Bioprints

Full Scale Human Neonatal Heart

Printed in LifeSupport

Heart Valve - Closed

Printed in LifeSupport

Heart Valve - Open

Printed in LifeSupport

Meniscus

Printed in LifeSupport

3D bioprinted heart with Lifeink 240 and LifeSupport

3D bioprinted heart with Lifeink 240 and LifeSupport

Product References

References for FRESH Printing:

Lee, A. et al. 3D bioprinting of collagen to rebuild components of the human heart. Science 365, 482–487 (2019).

Thomas, H. & Adam, F. 3D printing complex scaffolds using freeform reversible embedding of suspended hydrogels (FRESH). Frontiers in Bioengineering and Biotechnology4,(2016).

Isaacson, A., Swioklo, S. & Connon, C. J. 3D bioprinting of a corneal stroma equivalent. Experimental Eye Research173,188–193 (2018).

Jeon, O., Lee, Y., Hinton, T., Feinberg, A. & Alsberg, E. Cryopreserved cell-laden alginate microgel bioink for 3D bioprinting of living tissues. Materials Today Chemistry12,61–70 (2019).

Maxson, Eva L., et al. "In vivo remodeling of a 3D-Bioprinted tissue engineered heart valve scaffold." Bioprinting (2019): e00059.

Product Certificate of Analysis

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Safety and Documentation

Safety Data Sheet

Certificate of Origin

Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.