PhotoDextran®
Methacrylated Dextran
Catalog #5311, #5331, #5332, #5333
PhotoDextran®
Methacrylated Dextran
Catalog #5311, #5331, #5332, #5333
PhotoDextran® is 1 gram of lyophilized methacrylated dextran. PhotoDextran® provides 3D hydrogels that can be prepared at multiple concentrations and photocrosslinked (requires a photoinitiator) to provide various gel stiffness. Can be mixed with other photocrosslinkable ECM's.
Product Description
Advanced BioMatrix offers PhotoDextran®, a methacrylated dextran for photocrosslinkable hydrogels or incorporating within photocrosslinkable hydrogels (such as PhotoGel, PhotoCol or PhotoHA). PhotoDextran® provides native-like 3D gels with unique attributes: It can be prepared at various concentrations and photocrosslinked in a variety of way to provide a truly tunable gel stiffness. PhotoDextran® can be ordered by itself (1 gram lyophilized sterile powder), or as a kit that comes with a photoinitiator for photocrosslinking (see table below):
| Item | Catalog Number | Photoinitiator Included |
| PhotoDextran | #5311 | N/A |
| PhotoDextran with Irgacure | #5331 | Irgacure (365nm) |
| PhotoDextran with LAP | #5332 | LAP (405nm) |
| PhotoDextran with Ruthenium | #5333 | Ruthenium (400-450nm) |
Once solubilized, the PhotoDextran® can be stored at 2-10°C for 1 month.
| Parameter, Testing, and Method | Methacrylated Dextran #5311 |
| Sterilization Method | Filtration |
| Sterility - USP modified | No growth |
| Form | Lyophilized Powder |
| Package Size | 1 gram |
| Storage Temperature | -20°C |
| Shelf Life | Minimum of 6 months from date of receipt |
| Shelf Life After Reconstitution | 1 Month |
| Degree of Methacrylation | 45-65% |
| Molecular Weight | 35,000-50,000 Da |
| Endotoxin - IU/g | <10 |
Directions for Use
3D Hydrogel Preparation:
Note: Employ aseptic practices to maintain the sterility of the product throughout the preparation.
Recommended concentrations are 50-200 mg/ml
(5-20%).
Note: The following instructions are for a 5% hydrogel.
- Add 20 ml of 1X cold phosphate buffer saline (PBS) or cell culture media to the 1 gram of lyophilized methacrylated dextran powder.
- Mix on a shaker table or rotator plate until fully solubilized (~30 to 60 minutes) at 2-10°C.Note: Solubilization times may vary depending on the desired concentration and volume of PBS or medium added,
- Solubilize the photoinitiator at the recommended concentration, and add desired volume to your PhotoDextran®. See table below for concentration and volume recommendations.
- Add your cells to the solution if desired. Cells can also be added on top of crosslinked hydrogels.
- Dispense your PhotoDextran/photoinitiator/cell solution into the desired cultureware (i.e. 6-well plate, 48-well plate, etc...).
- Photocrosslink until a hydrogel of the desired stiffness has formed.
- Note: Longer exposure allows more crosslinking, though each cell type withstands different degrees of UV light and free radical exposure (generated by the photoinitiator) that mediates crosslinking.
| Photoinitiator | Photoinitiator Solubilization | Amount to add to PhotoDextran Solution |
| Irgacure | 100 mg/ml in Methanol | Multiply total volume of PhotoDextran by 0.01. Add this amount of Irgacure solution. |
| LAP | 17 mg/ml in 1X PBS or Media | Multiply total volume of PhotoDextran by 0.02. Add this amount of LAP solution. |
|
Ruthenium Sodium Persulfate |
Ruthenium - 37.4 mg/ml in 1X PBS or Media Sodium Persulfate - 119 mg/ml in 1X PBS or Media |
Multiply total volume of PhotoDextran by 0.02. Add this amount of both Ruthenium AND Sodium Persulfate solutions. |
NOTE: For LAP photoinitiator, the basic recommendations will result in a ~0.03% final LAP concentration. For some applications, such as DLP printing, the final LAP concentration should be increased to ~0.25-0.5% to accelerate and improve crosslinking.
Product Applications
The following graph shows the shear modulus G' (Pa) of PhotoDextran® at a 5% solution. The solution was prepared following the directions for use. Irgacure was used as the photoinitiator, and photocrosslinking was performed using 365nm light. The graph shows the hydrogel strength over time throughout continuous photocrosslinking of the solution.
Product Certificate of Analysis
Safety and Documentation
Product Disclaimer
This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
