Product Description

Advanced BioMatrix offers PhotoGel®-INK, a sterile, pre-formulated 10% methacrylated gelatin ink with 0.25% LAP for rapid 3D bioprinting with the BIONOVA X. 

The PhotoGel® consists of purified methacrylated porcine gelatin (25 mL) and LAP photoinitiator. The gelatin is type A 300 bloom.  

PhotoGel® is produced from methacrylated gelatin where the gelatin has been modified by reacting the free amines, primarily the ε-amines groups of the lysine residues as well as the a-amines groups on the N-termini. ~95% of the total lysine residues of the gelatin molecule have been methacrylated. 

Parameter, Testing, and Method Methacrylated Gelatin #D16110025360
Sterilization Method Filtration
Sterility - USP modified No growth
Form Sterile Solution
Package Size 25 mL
Storage Temperature 2-8°C
Shelf Life Minimum of 6 months from date of receipt
Degree of Methacrylation ~95%


Type A, 300 Bloom, Porcine Gelatin

Hydrogel Young's Modulus E (Pa)


Directions for Use

Directions for Use

Product Q & A

You can digest photocrosslinked methacrylated gelatin with Proteinase K or high temperatures, though these steps would likely kill cells in the process. The alternative is collagenase and trpysin, though higher concentrations may be required than standard protocols due to the photocrosslinked nature of the hydrogel. 

The PhotoGel is a type A, 300 bloom gelatin from porcine and has a molecular weight distribution that averages between 50-100 kDa. 

Product Applications

Read our Methacrylated Extracellular Matrices eBrochure Here

PhotoGel® Gelatin methacrylate can be used to form cross-linked hydrogels for tissue engineering[1] and 3D printing. The common forms of 3D printing using Lifeink® 300 include extrusion[2][3][4], inkjet[5] and photolithography[6]).

PhotoGel® has been used for endothelial cell morphogenesis,[7] cardiomyocytes,[8] epidermal tissue[9], injectable tissue constructs[10], bone differentiation[11], and cartilage regeneration[12].

Gelatin methacrylate has been explored in drug delivery applications in the form of microspheres[13] and hydrogels[14].



Product References

References for PhotoGel®:


Rothrauff, Benjamin B., et al. "Efficacy of thermoresponsive, photocrosslinkable hydrogels derived from decellularized tendon and cartilage extracellular matrix for cartilage tissue engineering." Journal of tissue engineering and regenerative medicine 12.1 (2018): e159-e170.

Rothrauff, Benjamin B., et al. "Anatomical region-dependent enhancement of 3-dimensional chondrogenic differentiation of human mesenchymal stem cells by soluble meniscus extracellular matrix." Acta biomaterialia 49 (2017): 140-151.

Rothrauff, Benjamin B., Guang Yang, and Rocky S. Tuan. "Tissue-specific bioactivity of soluble tendon-derived and cartilage-derived extracellular matrices on adult mesenchymal stem cells." Stem cell research & therapy 8.1 (2017): 133.

Bridge, Jack Christopher, et al. "Electrospun gelatin-based scaffolds as a novel 3D platform to study the function of contractile smooth muscle cells in vitro." Biomedical Physics & Engineering Express 4.4 (2018): 045039.

Capella-Monsonís, Héctor, et al. "Scaffolds for tendon tissue engineering." Handbook of Tissue Engineering Scaffolds: Volume One. Woodhead Publishing, 2019. 259-298.

Product Certificate of Analysis

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Safety and Documentation


Certificate of Origin

Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.