Product Description

Fibronectin is a widely used broad range natural cell attachment factor. This product has been purified from human plasma where it is found as a dimer and has a size of 440-500 kDa with two similar subunits (220-250 kDa ) linked by two disulfide bonds. Fibronectin’s primary function is related to cell adhesion and attachment to extracellular matrix. Certain domains of Fibronectin have been found to play important roles that involve interactions with collagen, heparin and other cell surface glycosaminoglycans (GAGs).
 
Fibronectin is ideal for coating of surfaces and is typically used at a concentration  of 1-5 ug/cm2 . It is provided in user-friendly packaging for use and storage. Fibronectin is sterile filtered and is supplied as a ready to use solution after thawing and concentration adjustment. This product is shipped separately on dry ice.
 
Parameter, Testing, and Method Fibronectin #5050
Quantity 1.0 mg
Volume 2.0 mL
Concentration 0.5 mg/mL
Purity - SDS PAGE Electrophoresis >95%
Formulation 20 mM Tris-HCL, pH 7.0 containing 0.45 M NaCl and 12% Glycerol
Form Solution
Source Human, Plasma
Storage Temperature -20°C
Shelf Life Minimum of 6 months from date of receipt
Sterilization Method Filtration
Cell Attachment Assay Passes
Sterility - USP modified No growth
Safety Source material found negative for infectious agents

Directions for Use

Recommended Volumes for 2D Coatings or 3D Hydrogels

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Coating Procedures:

Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.

  1. Thaw Fibronectin and dilute to desired concentration using serum-free medium or PBS (Ca++, Mg++ free). The final solution should be sufficiently dilute so that the volume added covers the surface evenly.
  2. Add appropriate amount of diluted material to culture surface.
  3. Incubate at room temperature for approximately 1 hour.
  4. Aspirate remaining material.
  5. Rinse plates carefully with dH2O– avoid scratching bottom surface of plates.
  6. Plates are ready for use. They may also be stored at 2-8°C damp or air dried if sterility is maintained.

Product Q & A

440 kDa. The protein is a dimer of two 220 kDa identical subunits. It shows up as 220 kDa on a reduced gel, and 440 kDa on a non-reduced gel.

The fibronectin (Cat. No. 5080-5MG) is prepared in a glycine/NaCl buffer and when the lyophilized pellet is reconstituted with sterile water at a 1 mg/ml concentration the resulting pH is neutral (~7.4-7.6).

Fibronectin has two nearly identical molecules that are linked together in a dimer configuration. Each of those molecules has one RGD site: https://doi.org/10.1002/iub.493

Product Applications

Matrigel Alternatives for Neuronal Assays

Matrigel Alternatives for Migration and Wound Healing Assays

Product References

References for Fibronectin:

Alhussein, Ghada, et al. "A spatiotemporal characterization method for the dynamic cytoskeleton." Cytoskeleton 73.5 (2016): 221-232.

Pezzoli, Daniele, et al. "Fibronectin promotes elastin deposition, elasticity and mechanical strength in cellularised collagen-based scaffolds." Biomaterials 180 (2018): 130-142.

Wiley, Luke A., et al. "Generation of xeno‐free, cGMP‐compliant patient‐specific iPSCs from skin biopsy." Current protocols in stem cell biology 42.1 (2017): 4A-12.

Wiley, Luke A., et al. "Using patient-specific induced pluripotent stem cells and wild-type mice to develop a gene augmentation-based strategy to treat CLN3-associated retinal degeneration." Human gene therapy 27.10 (2016): 835-846.

Worthington, Kristan S., et al. "Two-photon polymerization for production of human iPSC-derived retinal cell grafts." Acta biomaterialia 55 (2017): 385-395.

Li, Ling, Mizuho Fukunaga-Kalabis, and Meenhard Herlyn. "Isolation and cultivation of dermal stem cells that differentiate into functional epidermal melanocytes." Human Cell Culture Protocols. Humana Press, 2012. 15-29.

Boularaoui, Selwa Mokhtar, et al. "Efficient transdifferentiation of human dermal fibroblasts into skeletal muscle." Journal of tissue engineering and regenerative medicine 12.2 (2018): e918-e936.

Product Certificate of Analysis

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Safety and Documentation

Safety Data Sheet

Certificate of Origin

Testing Exemption

Example Certificate of Analysis

Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.