Product Description

PEPTITE-2000® is a synthetic peptide containing the RGD cell attachment sequence found in fibronectin, vitronectin and many other matrix and serum proteins. This RGD peptide has been specifically engineered to spontaneously adhere to a variety of surfaces such as polystyrene (either tissue culture treated or non-treated), hollow fibers made of cellulose, glass, polycarbonate membranes and many other materials. This binding is mediated via a hydroscopic C-terminal sequence. The RGD motif is present at the N-terminal end of the peptide, allowing for optimal cell attachment via integrin receptors.

PEPTITE-2000® can be substituted as the primary cell attachment component in a chemically defined animal component-free culture system.  

Parameter, Testing, and Method

PEPTITE-2000®
Catalog # 5462-5MG

Form

Powder

Package Size

5 mg

Sterilization Method

Non-Sterile

Peptide Sequence

Ac-Gly-D-Arg-Gly-Asp-Ile-Pro-Ala-Ser-Ser-Lys-Gly-Gly-Gly-Gly-Ser-D-Arg-Leu-Leu- Leu-Leu- Leu-Leu-D-Arg-NH2

Counter Ion

Acetate

Purity Confirmed by RP-HPLC

>95%

Identity Confirmed by Mass Spectrometry

Characteristic

Cell Attachment Assay

Pass

Storage Temperature

 2-10°C

Shelf Life

Minimum of 6 months from date of receipt

PEPTITE-2000® is supplied in non-sterile 5 mg quantities.

Directions for Use

Use these recommendations as guidelines to determine the optimal coating conditions for your culture system. Two options are provided:

Procedure A

  1. Remove cap and add 5 mL of serum-free medium or PBS to the bottle.
  2. Replace cap and vortex contents vigorously. Ensure that the PEPTITE-2000® is completely solubilized.  The solution will remain slightly hazy.
  3. Transfer desired volume of solution from the bottle to a dilution vessel.  Dilute to desired concentration using serum-free medium or PBS.   A typical working concentration may range from 0.1 to 10 µg/mL.
  4. Sterile filter solution through a 0.22 micron button filter. 
  5. Aseptically add appropriate amount of diluted, sterile material to culture surface.
  6. Incubate at room temperature or 37°C, covered, for 1-2 hours.
  7. After incubation, aspirate remaining material.
  8. Rinse plates carefully with dH2O– avoid scratching bottom surface of plates.
  9. Plates are ready for use.  They may also be stored at 2-8°C damp or air dried if sterility is maintained.
  10. Store remaining solubilized PEPTITE-2000® at 2 to 10°C.

Additional note:  Include divalent cations (Calcium, Magnesium, or Manganese) in cell attachment solution to obtain optimum cell binding.

Procedure B

  1. Remove cap and add 5 mL of sterile 70% Ethanol
  2. Replace cap and vortex contents. Ensure that the PEPTITE-2000® is completely solubilized.
  3. Transfer desired volume of solution from the bottle to a dilution vessel.  Dilute to the desired concentration using 70% Ethanol.  Concentrations from 0.1 to 10 ug/mL should be tested.
  4. Add appropriate amount of diluted material to culture surface.
  5. Leave the coated container, uncovered, in a laminar flow hood until the wells are dry.
  6. Rinse plates carefully with dH2O– avoid scratching bottom surface of plates.
  7. Plates are ready for use.
  8. Store remaining solubilized PEPTITE-2000® at 2-10°C.

Product References

References for Peptite-2000®:

Geuss, Laura R., Scott Gorthey, and Laura J. Suggs. "Magnetic Attraction of Paramagnetic Beads Influences Lineage Commitment in Embryoid Bodies."

Geuss, Laura R., et al. "Paramagnetic beads and magnetically mediated strain enhance cardiomyogenesis in mouse embryoid bodies." PloS one 9.12 (2014): e113982.

Sanchez‐Rodriguez, Sandra P., et al. "Plasmonic activation of gold nanorods for remote stimulation of calcium signaling and protein expression in HEK 293T cells." Biotechnology and bioengineering 113.10 (2016): 2228-2240.

Saito, Masaya, et al. "Intestinal epithelial cells with impaired autophagy lose their adhesive capacity in the presence of TNF-α." Digestive diseases and sciences 57.8 (2012): 2022-2030.

Product Certificate of Analysis

No result for .

Safety and Documentation

Safety Data Sheet

Certificate of Origin

Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.