Product Description

CD164 (Sialomucins) belongs to a heterogeneous group of secreted or membrane-associated mucins that appear to play two key but opposing roles in vivo: 1) as cytoprotective or anti-adhesive agents and 2) as adhesion receptors. CD164 is a type I integral transmembrane sialomucin that functions as an adhesion receptor. Recombinant CD164 protein may serve as coating matrix protein for studying of hematopoietic stem cell (HSC) functions in vitro.

Full-length of human CD164 extracellular domain cDNA (24 – 162 aa) was constructed with 29 N-terminal T7/His tag and expressed in E. coli as inclusion bodies.  

The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified as soluble protein.

Parameter, Testing, and Method CD164 #5100
Quantity 0.05 mg
Volume 0.1 mL
Concentration 0.5 mg/mL
Purity - SDS PAGE Electrophoresis > 90%
Formulation Formulated in 20 mM pH 8.0 TRIS-HCL Buffer, with proprietary formulation of NaCl, KCl, EDTA, L-Arginine, DTT and Glycerol
Form Solution
Production Type Recombinant - E. Coli
Storage Temperature -20°C
Shelf Life Minimum of 6 months from date of receipt
Sterilization Method Filtration
Cell Assay Pass
Sterility - USP modified No Growth
Accession Number NP_006007
Recombinant Protein Sequence MASMTGGQQMGRGHHHHHHGNLYFQGGEFDKNTTQHPNVTTLAPI
SNVTSAPVTSLPLVTTPAPETCEGRNSCVSCFNVSVVNTTCFWIE
CKDESYCSHNSTVSDCQVGNTTDFCSVSTATPVPTANSTAKPTVQ
PSPSTTSKTVTTSGTTNNTVTPTSQPVRKSTFD

Directions for Use

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Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.

  1. Thaw CD164 and dilute to desired concentration using serum-free medium or PBS. The final solution  should be sufficiently dilute so that the volume added covers the surface evenly.  Note: Use 1 ml PBS per well in a 6-well plate.
  2. Add 1 – 10 µg protein to each well and incubate at 2 to 10°C overnight.
  3. After incubation, aspirate remaining material.
  4. Plates are ready for use. They may also be stored at 2-8°C damp or air dried if sterility is maintained.

Coating this recombinant matrix protein at 1-10 ug / well (6 well plate) in HSC cell specific medium can be used for human HSC / receptor interaction study in vitro.

Product Certificate of Analysis

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Safety and Documentation

Safety Data Sheet

Certificate of Origin

Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.