Product Description

Poly-D-Lysine is a synthetic amino acid chain that is positively charged and widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces.  This molecule is resistant to enzymatic degradation and has been used to culture a wide variety of cell types.

The molecular weight of Poly-D-Lysine can vary significantly with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (>300,000 Da) having more binding sites per molecule.  This product’s molecular weight ranges from 70,000 to 150,000 Da yielding a solution viscosity for easy handling while providing sufficient binding sites for cell attachment.

Poly-D-Lysine surface coatings are designed to improve cell attachment, growth and differentiation of many cell types.  Coated surfaces will often improve cell attachment in reduced or serum-free conditions.  Poly-D-Lysine is supplied in a sterile 5 mg package size.

Parameter, Testing, and Method Poly-D-Lysine #5049
Form Solution
Package Size 50 mL
Storage Temperature 2-10°C
Sterilization Method Filtration
Molecular Weight 70,000 - 150,000 Da
Sterility - USP modified No Growth
Cell Attachment Assay Pass
Source Synthetic
Shelf Life Minimum of 6 months from date of receipt
Concentration 0.1 mg/mL

 

 

Directions for Use

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Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.  To maintain sterility, perform all operations in a laminar flow hood.

  1. A typical working concentration is 0.1 mg/mL. If a different concentration is desired, transfer desired volume of solution from the bottle to a dilution vessel.  Dilute to desired concentration using tissue culture grade water or PBS.
  2. Add appropriate amount of diluted material to culture surface. Typically, 1 mL per 25 cm2 is used. Rock gently to ensure uniform coating of culture surface.
  3. After 5 minutes, remove excess solution by aspiration.
  4. Thoroughly rinse surface with tissue culture grade water.
  5. Incubate and allow to dry at room temperature or 37°C, covered, for at least 2 hours.
  6. Introduce medium and cells to the culture surface.
  7. Store remaining Poly-D-Lysine at 2 to 10°C.

Product References

References for Poly-D-Lysine:

Albuquerque, Cristóvão, et al. "Preparation of coverslips for neuronal cultures." Cold Spring Harbor Protocols 2009.8 (2009): pdb-prot5272.

Unsain, Nicolas, et al. "Production and isolation of axons from sensory neurons for biochemical analysis using porous filters." JoVE (Journal of Visualized Experiments) 89 (2014): e51795.

Li, Ling, Mizuho Fukunaga-Kalabis, and Meenhard Herlyn. "Isolation, characterization, and differentiation of human multipotent dermal stem cells." Skin Stem Cells. Humana Press, Totowa, NJ, 2013. 235-246.

Baik, Matthew, et al. "Identification of invadopodia by TKS5 staining in human cancer lines and patient tumor samples." MethodsX 6 (2019): 718-726.

Tammia, Markus, et al. "Egr2 overexpression in Schwann cells increases myelination frequency in vitro." Heliyon 4.11 (2018): e00982.

Boularaoui, Selwa Mokhtar, et al. "Efficient transdifferentiation of human dermal fibroblasts into skeletal muscle." Journal of tissue engineering and regenerative medicine 12.2 (2018): e918-e936.

Johnstone, Aaron D., et al. "Developmental axon degeneration requires TRPV1-dependent Ca2+ influx." eNeuro 6.1 (2019).

Product Certificate of Analysis

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Safety and Documentation

Safety Data Sheet

Certificate of Origin

Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.