Product Description

ECM Select® Array Kit Ultra-36 is an extracellular matrix screening array. This product has thirty six (36) human extracellular matrix protein (ECM) conditions that are deposited onto the hydrogel surface as printed array spots.  Each ECM condition is printed with nine (9) replicate spots where each spot has diameter of 400 µm.  The ECMs are localized within each spot without cross diffusion from neighboring spots, therefore each spot represents an independent ‘well’ or experiment.

ECM Select Array Kit Ultra MCF7 and Hepatocytes

The ECM Select® Array Kit Ultra-36 contains a microscope glass slide printed with the ECM conditions contained within a protective slide holder and a pre-sterilized tray.  The components are sterilized and ready-to-use.

Cells of interest are seeded on to the ECM Select® Array slide and allowed to be cultured in the incubator for the desired amount of time. Cell morphology, attachment and growth can be visualized using a bright field microscope. Specific cellular behaviors can be monitored by staining the cells on the slide using specific fluorescence based marker. Fluorescence signal can be detected using fluorescence microscope imaging system.

The ECM Select® Array Kit Ultra-36 is simple to use in comparison to performing the same experiment in multi-well tissue culture plates.  Cell seeding, fixing, washing and process steps are done in one single solution exchange, eliminating multi-well solution handlings. From start to finish, the optimal ECM for culturing cells can be determined in less than 24 hours for most cell types.

Product Q & A

Because it is contact printing, it is hard to determine the the precise amount. Based on protein staining result, each spot is about 0.1-1ng.

There may be possibility of antagonist effect of two different component. We have not seen this very often, what we usually see is one of the component by itself does not provide adhesion effect, but when combine with another ECM, greatly enhance the other ECM.

The stiffness is ~10 kPa.

Mounting media tends to destroy the polyacrylamide hydrogel that the ECM spots are printed onto. The best method we have found is to incubate the entire slide  for 20 minutes with 4% PFA prior to the stain protocol. The slide was cover-slipped with a 90% glycerol medium containing the PPD anti-fade compound and sealed with fingernail polish around the edges.

Product References

References for ECM Select®:

Shaheen, Noel L., et al. "Extracellular matrix composition modulates angiosarcoma cell attachment and proliferation." Oncoscience11-12 (2017): 178.

Ferreira, L. P., V. M. Gaspar, and J. F. Mano. "Design of Spherically Structured 3D In vitro Tumor Models-Advances and Prospects." Acta biomaterialia(2018).

Product Certificate of Analysis

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Safety and Documentation

Safety Data Sheet

Certificate of Origin

Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.