Product Description

ECM Select^® Array Kit Ultra-36 is an overnight extracellular matrix screening array. This product has thirty six (36) human extracellular matrix protein (ECM) conditions that are deposited onto the hydrogel surface as printed array spots.  Each ECM condition is printed with nine (9) replicate spots where each spot has diameter of 200-400 µm.  The ECMs are localized within each spot without cross diffusion from neighboring spots, therefore each spot represents an independent ‘well’ or experiment.The slide is intended for evaluation of initial attachment (<24 hours). Long term culture may cause the spots to separate or the polyacrylamide coating to detach.

The ECM Select^® Array Kit Ultra-36 contains a microscope glass slide printed with the ECM conditions contained within a protective slide holder and a pre-sterilized tray.  The components are sterilized and ready-to-use.

Cells of interest are seeded on to the ECM Select^® Array slide and allowed to be cultured in the incubator for the desired amount of time. Cell morphology, attachment and growth can be visualized using a bright field microscope. Specific cellular behaviors can be monitored by staining the cells on the slide using specific fluorescence based marker. Fluorescence signal can be detected using fluorescence microscope imaging system.

The ECM Select^® Array Kit Ultra-36 is simple to use in comparison to performing the same experiment in multi-well tissue culture plates.  Cell seeding, fixing, washing and process steps are done in one single solution exchange, eliminating multi-well solution handlings. From start to finish, the optimal ECM for culturing cells can be determined in less than 24 hours for most cell types.