Collagen Fiber Diameter and Pore Size
08/26/19
First Publication Abstract:
We utilized polymerization temperature (4–37°C) to manipulate collagen hydrogel fiber diameter, space-filling properties, and cross-link density. Multiphoton microscopy and scanning electron microscopy reveal that as polymerization temperature decreases:
- Fiber diameter increases
- Pore size increases
- Hydrogel storage modulus decreases
- Second harmonic generation decreases
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Second Publication Abstract:
In this study, FAPs’ sensitivity to their ECM substrate was assessed using collagen coated polyacrylamide to control substrate stiffness and collagen hydrogels to engineer concentration, crosslinking, fibril size, and alignment. FAPs on substrates of fibrotic stiffnesses had increased myofibroblast activation, depicted by αSMA expression, compared to substrates mimicking healthy muscle, which correlated strongly YAP nuclear localization.
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