How to Make 3D Collagen Hydrogels
This video explains and shows how to effectively and efficiently form a 3D collagen gel using Type I collagen from Advanced BioMatrix. The 4 main requirements for a successful collagen hydrogel are:
1. Neutral pH (7.0-7.4)
2. Isotonic Salts (~300 mOsmo)
3. Temperature (37°C)
4. Concentration (>1.5 mg/ml)