Airway Epithelial Cystic Fibrosis Model
06/03/20
Publication Title:
GGC, a Novel Prophylactic and Therapeutic Anti-oxidative Treatment for Cystic Fibrosis
Advanced BioMatrix Products Used:
Type I Collagen Hydrogels eBrochure
How the products were used:
PureCol® type I collagen was used to create a 3D air-liquid interface differentiated epithelial cell culture.
Article Abstract:
Proteomic analysis identified perturbation of several pathways related to cellular respiration and stress responses upon LPS challenge. Most of these were resolved when cells were treated with GGC. While GGC did not resolve LPS-induced IL-8 and IDO-1 activity, it effectively attenuated LPS-induced ROS and stress granule formation, while significantly increasing intracellular glutathione levels and improving epithelial cell barrier integrity. Moreover, we compared the effect of GGC with thiols NAC and glutathione on cell viability. GGC was the only thiol that increased cell viability; protecting cells against LPS induced cell death. Both therapeutic and prophylactic treatments were successful.
