Mechanical Actions Between Cancer and Collagen
09/09/20
Publication Title:
Fibroblast Promotes Head and Neck Squamous Cell Carcinoma Cells Invasion Through Mechanical Barriers in 3D Collagen Microenvironments
Advanced BioMatrix Products Used:
FibriCol® 10 mg/ml Type I Atelocollagen Solution.
How the products were used:
FibriCol® 10 mg/ml Type I Atelocollagen Solution was neutralized and diluted to concentrations of 1, 2, 4 and 8 mg/ml, creating 3D collagen hydrogels of various storage moduli (stiffness). Results showed that stiffer 3D matrix, with smaller pore size, limited force-induced collagen remodeling and invasion of OECM-1 spheroids. In contrast, in co-culture spheroids, the HDF cells could remodel the collagen matrices, via MMPs mediated collagen degradation, to increase the invasion capability of OECM-1 cells.
Article Abstract:
We co-cultured head-and-neck squamous cell carcinoma (OECM-1 cells) and human dermal fibroblasts (HDF) to form 3D spheroids, embedded in collagen gel with different concentrations to delineate their roles and their interactions in cancer cell invasion. We showed that, in single-species spheroids, the OECM-1 cells could not remodel the high concentration (8 mg/mL) collagen matrices to invade into the surrounding collagen. In contrast, in the co-culture spheroids, the HDF cells could remodel the collagen matrices, via MMPs meditated collagen degradation, to increase the invasion capability of OECM-1 cells.
