Collagenase

Yes.

There are sufficient levels of divalent cations in the lyophilized collagenase powder to achieve full enzymatic activity. Therefore, solubilizing the lyophilized collagen powder in medium or a phosphate buffered solution without divalent cations is recommended per the Directions for Use.

Collagenase is most stable in the lyophilized form stored at 2 to 10°C.  After reconstitution, it is recommended that any remaining solubilized collagenase be aliquoted into desired volumes and then frozen at < -20°C  for up to 1 to 2 months.  Repeat freezing and thawing is not recommended.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4698262/