Culturing Single Cells in 3D
07/21/20
Publication Title:
Deterministic Culturing of Single Cells in 3D
Advanced BioMatrix Products Used:
PhotoCol® Methacrylated Type I Collagen
How the products were used:
Our methacrylated type I collagen was used to form mini "3D hydrogel islands." Cells were individually added to each "island" in order to culture them in relative isolation from the rest of the cell population.
Article Abstract:
Using examples of breast and colorectal cancers, we show that individual cells evolve into tumors or aspects of tumors displaying different characteristics of the initial cancer type and aggressiveness. By implementing a morphometry assay with luminal A breast cancer, we demonstrate the potential of the proposed approach to study phenotypic heterogeneity. Results reveal that intertumor heterogeneity increases with time in culture and that varying degrees of intratumor heterogeneity may originate from individually seeded cells.
Link to the publication:
https://www.nature.com/articles/s41598-020-67674-3?elqTrackId=a42e833ca22544b9811a53a9a5b90abc
