HyStem Hydrogel Stiffness
02/07/20
In this white paper, we measure the hydrogel stiffness of our three main HyStem kits, including HyStem, HyStem-C and HyStem-HP.
Mechanical stiffness is a critical parameter to consider when designing a cell culture system. In the context of 3D cell culture, matrix stiffness can impact a wide variety of pathways including stem cell differentiation, cancer metastasis, and cardiac tissue function.
HyStem kits are thiolated hyaluronic acid that can form 3D hydrogels at room temperature via chemical crosslinking after the kit components have been mixed together. Advanced BioMatrix also offers PhotoHA methacrylated hyaluronic acid kits which form 3D hydrogels using photocrosslinking.
Data was collected using an Elastosens rhometer from Rheolutions www.rheolution.com.
