Tropoelastin

E. Coli

Tropoelastin is produced from full length sequence as a full-length protein. Tropoelastin is the soluble pre-cursor to elastin as it is made in vivo. In the body, a leader sequence is cleaved off of tropoelastin which takes it from a soluble format inside the cell to a solid cross-linked elastin fibrous protein. One major advantage of being able to work with a full length tropoelastin is that it retains much of its native properties, far more than any peptide sequence.

Yes – there is a poly-His tag at the carboxy terminus.

No.

The tropoelastin is soluble in water at a pH below 6.5 to 7 and at temperatures below 30°C. Tropoelastin will tend to precipitate at higher pH and temperatures approaching 37°C. Solubility in water improves with lower pH. Tropoelastin is soluble in acetic acid.

Tropoelastin will form a stable solution at a concentration of up to 100 mg/ml.

Tropoelastin is soluble in butanol and hexafluoro propanol.