3D Bioprinting Nasal Cartilage
02/24/21
Publication Title:
Comparison of Annulus Fibrosus Cell Collagen Remodeling Rates in a Microtissue System
Advanced BioMatrix Products Used:
Lifeink® 200 35 mg/ml type I Collagen Bioink
How the products were used:
Human nasoseptal chondrocytes were mixed with Lifeink 200 collagen bioink and 3D bioprinted. In vitro chondrogenesis resulted in engineered nasal cartilage constructs akin to native human nasal cartilage.
Article Conclusion:
The engineered nasal cartilage constructs displayed molecular, biochemical and histological characteristics akin to native human nasal cartilage. In this work, we report a successful in vitro nasal cartilage tissue engineering method via the FRESH 3D bioprinting. In brief, a commercially available type I collagen hydrogel (Lifeink 200) was premixed with monolayer‐expanded human nasoseptal chondrocytes (hNCs) to create an hNCs‐laden construct for the bio‐fabrication of tissue‐engineered nasal cartilages. The effect of the addition of nasal chondrocytes on the hydrogel's rheological properties and printability were also investigated. The hNCs‐laden constructs were 3D printed, cultured in chondrogenic media, and the resulting engineered tissue's structural integrity was evaluated along with biochemical and molecular assessments.
Link to the publication:
https://faseb.onlinelibrary.wiley.com/doi/full/10.1096/fj.202002081R
