Lifeink 200 with Optical Coherence Tomography
12/29/21
Publication Title:
In Situ Volumetric Imaging and Analysis of FRESH 3D Bioprinted Constructs Using Optical Coherence Tomography
Advanced BioMatrix Products Used:
Lifeink® 200 35 mg/ml type I Collagen Bioink
How the products were used:
Lifeink 200 collagen bioink was 3D bioprinted using FRESH printing. Authors then used an integrated optical coherence tomography (OCT) for in situ volumetric imaging, error detection, and 3D reconstruction.
![](assets/uploads/printed_defect_oct.jpg)
Article Conclusion:
Authors report "improvements to the Freeform Reversible Embedding of Suspended Hydrogels (FRESH) bioprinting process through new collagen bioink compositions, support bath optical clearing, and machine pathing. This enables high-fidelity 3D volumetric imaging with micron scale resolution over centimeter length scales, the ability to detect a range of print defect types within a 3D volume, and real-time imaging of the printing process at each print layer."
Link to the publication:
https://www.biorxiv.org/content/10.1101/2021.06.30.450389v2