Matrix Stiffness Affects Breast Carcinoma

Matrix Stiffness Affects Breast Carcinoma


Publication Title:

Machine Learning Reveals Mesenchymal Breast Carcinoma Cell Adaptation in Response to Matrix Stiffness


Advanced BioMatrix Products Used: 

CytoSoft® PDMS Soft Substrate Plates

ECM Select® Screening Array

VitroCol® Human Type I Collagen 

CytoSoft eBrochure


How the products were used:

CytoSoft® Plates (soft substrate 6-well plates with a thin layer of PDMS of a specific stiffness, including 0.2, 0.5, 2, 8, 16, 32 and 64 kPa) were used to evaluate the affect of matrix stiffness on mesenchymal breast cancer cells. The ECM Select® was used to determine the optimal extracelluar matrices for coating the plates. For this study, VitroCol® human type I collagen was determined to be the best substrate for cell attachment. 



Article Conclusion:

"We found that mesenchymal breast cancer cells responded to different substrate stiffness by morphological adaptation, changes in proliferation rate and cytoskeleton markers such as cell-cell adhesion protein E-cadherin. Surprisingly, the cell properties on the stiffest tested substrate that mimicked bone stiffness changed dramatically–they formed multicellular clusters with distinct E-cadherin localisation at the cell-cell adhesion surfaces. Our results suggest that the stiffest microenvironment can induce cell transitioning from mesenchymal to epithelial cell phenotype."


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