Nutragen Collagen Bioink
06/04/21
Publication Title:
3D Bioprinting and Microscale Organization of Vascularized Tissue Constructs using Collagen-Based Bioink
Advanced BioMatrix Products Used:
Nutragen® 6 mg/ml type I Atelocollagen
Advanced BioMatrix Collagen eBrochure
How the products were used:
Nutragen® highly purified type I collagen was mixed with Xanthan Gum and endothelial cells to bioprint a 3D matrix. The shape and ECM components of the construct promoted vessel formation. Post‐print culture of the bioprinted tissues resulted in endothelial sprouting and formation of interconnected capillary‐like networks within the lattice pattern and between the fibroblast layers.
Article Conclusion:
"Using a commercial extrusion‐based multi‐head bioprinter and collagen‐ XG bioink, the component cells were spatially assembled, wherein the endothelial cells were bioprinted in a lattice pattern and sandwiched between bioprinted fibroblasts layers. 3D bioprinted constructs thus generated were stable, and maintained structural shape and form. Post‐print culture of the bioprinted tissues resulted in endothelial sprouting and formation of interconnected capillary‐like networks within the lattice pattern and between the fibroblast layers. Bioprinter‐assisted spatial placement of endothelial cells resulted in fabrication of patterned prevascularized constructs that enable potential regenerative applications in the future."
