Rat Tail Collagen to Study Pulmonary Fibrosis
04/07/21
Publication Title:
A Novel Fibroblast Reporter Cell Line for In Vitro Studies of Pulmonary Fibrosis
Advanced BioMatrix Products Used:
RatCol® Rat Tail Collagen, 4 mg/ml, #5153-1KIT
How the products were used:
Our Rat Tail Collagen was used in various assays using a novel fibroblast reporter cell line in in vitro studies of pulmonary fibrosis.
Article Conclusion:
"We report the generation of a representative fibroblast reporter cell line (10-4ABFP) to study pathophysiological mechanisms of IPF in high throughput or high resolution in vitro live cell assays. To this end, we immortalized primary fibroblasts isolated from the distal lung of Sprague-Dawley rats. Molecular and transcriptomic characterization identified clone 10-4A as a matrix fibroblast subpopulation. Mechanical or chemical stimulation induced a reversible fibrotic state comparable to effects observed in primary isolated fibroblasts."
Link to the publication:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582034/
