Stiffness Effects on DNA Methylation
01/23/24
Publication Title:
Substrate Elasticity does not Impact DNA Methylation Changes During Differentiation of Pluripotent Stem Cells
Advanced BioMatrix Products Used:
CytoSoft® PDMS Soft Substrate Plates
How the products were used:
CytoSoft® Plates (soft substrate 6-well plates with a thin layer of PDMS of a specific stiffness, including 0.2, 0.5, 2, 8, 16, 32 and 64 kPa) were used to evaluate the affect of matrix stiffness on iPSC differentiation and epigenetic modification of DNA methylation.
Article Conclusions:
Stiffness of PDMS-substrates did not impact on directed differentiation of iPSCs, whereas the moderate epigenetic differences on TCP might also be attributed to other chemical parameters.
Link to the publication:
https://www.biorxiv.org/content/10.1101/2024.01.16.575833v1.full
