Substrate Stiffness on Vaginal Fibroblasts
Extracellular Matrix Stiffness and Composition Regulate the Myofibroblast Differentiation of Vaginal Fibroblasts
Advanced BioMatrix Products Used:
How the products were used:
CytoSoft® Plates (soft substrate 6-well plates with a thin layer of PDMS of a specific stiffness, including 0.2, 0.5, 2, 8, 16, 32 and 64 kPa) were used to evaluate the affect of environmental substrate stiffness on vaginal fibroblast to myofibroblast differentiation.
Gene expression levels of ACTA2 steadily increased from 0.2 kPa to 8 kPa. This study also showed that a combination of ECM and substrate stiffness are required to regulate myofibroblast differentiation.
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