Substrate Stiffness on Vaginal Fibroblasts
06/10/21
Publication Title:
Extracellular Matrix Stiffness and Composition Regulate the Myofibroblast Differentiation of Vaginal Fibroblasts
Advanced BioMatrix Products Used:
CytoSoft® PDMS Soft Substrate Plates
How the products were used:
CytoSoft® Plates (soft substrate 6-well plates with a thin layer of PDMS of a specific stiffness, including 0.2, 0.5, 2, 8, 16, 32 and 64 kPa) were used to evaluate the affect of environmental substrate stiffness on vaginal fibroblast to myofibroblast differentiation.
Article Conclusion:
Gene expression levels of ACTA2 steadily increased from 0.2 kPa to 8 kPa. This study also showed that a combination of ECM and substrate stiffness are required to regulate myofibroblast differentiation.
Link to the publication:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7369731/
