Substrate Stiffness to Study Cardiomyocytes
06/10/21
Publication Title:
Decreased YAP Activity Reduces Proliferative Ability in Human Induced Pluripotent Stem Cell of Duchenne Muscular Dystrophy Derived Cardiomyocytes
Advanced BioMatrix Products Used:
CytoSoft® PDMS Soft Substrate Plates
How the products were used:
CytoSoft® Plates (soft substrate 6-well plates with a thin layer of PDMS of a specific stiffness, including 0.2, 0.5, 2, 8, 16, 32 and 64 kPa) were used to evaluate the nuclear/cytoplasmic ratio in induced pluripotent stem cell derived cardiomyocytes across a broad range of soft and hard substrates.
Article Conclusion:
The results indicate that in substrate stiffness which ranged from 0.5 to 32 kPa using biocompatible silicone, hard substrate (32 kPa) showed higher N/C ratio than soft substrate (0.5 kPa) in Con-iPSC-CMs
Link to the publication:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121946/
