AlamarBlue Assay Protocol
08/27/24
The alamarBlue assay is a widely used and versatile method for assessing cell viability and proliferation. It is based on the reduction of the non-fluorescent compound resazurin to the fluorescent compound resorufin by metabolically active cells. This reduction process indicates the presence of living cells and can be quantitatively measured using fluorescence or absorbance techniques.
The alamarBlue assay is non-toxic, simple and highly sensitive, capable of detecting even small numbers of viable cells. This assay has been widely employed to characterize cell growth in various 3D cell culture platforms. Here we are using the alamarBlue assay to assess the proliferation of cells cultured in our PureCol® collagen hydrogels.
Download the AlamarBlue Assay for Testing Cell Proliferation on Encapsulated Cells Protocol.
