Immunofluorescence Staining Protocol
08/27/24
Immunofluorescence staining or immunocytochemistry is a laboratory technique to detect specific proteins or antigens expression in cells with the aid of selective probes that can be visualized under a fluorescence microscope.
This protocol outlines a two-step ICC process, employing a primary antibody targeting a specific antigen and a secondary antibody labeled with a fluorescent probe. The following procedure has been adapted for 3D bioprinted samples containing cells encapsulated in a hydrogel, which have already been prepared (ie. paraffin or frozen).
