Product Description

Cytus™ Violet Cell Adhesion Stain is a ready-to-use crystal violet-based staining solution designed for visualization and quantification of adherent cells. The dye binds strongly to cellular DNA and proteins, producing an intense, uniform stain that highlights attached cells while nonadherent cells are removed during washing.

This enables clear evaluation of cell attachment, spreading, and adhesion performance across a wide range of substrates, including tissue culture plastics, extracellular matrix coatings, and stiffness-tunable biomaterials such as CytoSoft™.

The workflow is simple and reproducible, supporting both imaging-based analysis and quantitative plate-based measurements. When combined with automated imaging or plate readers, Cytus™ Violet enables efficient, high-content analysis of cell adhesion across multiple conditions.

Parameter, Testing, and Method Cytus™ Violet #5466
Dye Type Crystal Violet
Stock Concentration 0.1% w/v in water
Stock Volume 2 x 25 mL
Appearance Dark purple liquid
Sterility Sterile
Assay Type Cell Adhesion
Compatibility Binds strongly to DNA and proteins
Working Concentration 0.1% w/v
Working Volume / Well (Plate: 96-well) 100 to 200 µL
Detection Methods Brightfield and fluorescence
Absorbance ~590 nm
Fluorescence Excitation/Emission (optional) ~592/636 nm
Storage Room temperature (25°C)
Shelf Life Minimum of 6 months from date of receipt

 

Directions for Use

Directions for Use

Product Applications

  • Cell adhesion assays

  • Proliferation and endpoint cell quantification

  • Biomaterial and ECM coating evaluation (PureCol®, GelMA, etc.)

  • Mechanobiology and stiffness studies (CytoSoft™ plates)

  • High-throughput screening (96-well and higher formats)

  • Cell spreading and morphology analysis

Product Certificate of Analysis

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Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.