Product Description

Cytus™ Blue is a ready-to-use nuclear staining solution based on Hoechst 33342, a bis-benzimide dye that binds to the minor groove of DNA. It produces bright blue/cyan fluorescence (Ex/Em ~350/454 nm), enabling high-contrast visualization of nuclei across a wide range of mammalian cell types.

The dye is membrane permeable, allowing efficient staining of both live and fixed cells. Cytus™ Blue integrates seamlessly into fluorescence microscopy, plate-based assays, and flow cytometry workflows, making it a flexible tool for imaging and quantitative analysis.

Parameter, Testing, and Method Cytus™ Blue #5469
Nuclear Dye Blue (Cytus™ Blue Hoechst-33342)
Stock Concentration 1 mM in water
Stock Volume 100 µL
Appearance Green hue, clear liquid
Sterility Non-Sterile
Assay Type Nuclear Stain
Cell Compatibility Live and fixed cells
Working Concentration 1–5 µM
Working Volume / Well (Plate: 96-well) 100 to 200 µL
Excitation/Emission Blue ~350 / 454 nm
Storage Temperature -20°C
Shelf Life Minimum of 6 months from date of receipt

 

Product Applications

  • Live-cell imaging

  • Fixed-cell fluorescence microscopy

  • High-content screening (HCS)

  • Cell counting and normalization assays

  • Flow cytometry (DNA staining)

  • Co-staining with viability or cytoskeletal dyes

Product Certificate of Analysis

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Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.