Product Description

The Cytus™ Green / Red Cell Viability Kit is a dual-color fluorescence assay designed for rapid and reliable assessment of live and dead cells. It combines Cytus™ Green Calcein AM for viable cells with Cytus™ Red Propidium Iodide for non-viable cells, enabling simultaneous discrimination within mixed populations.

Cytus™ Green is a membrane-permeable, non-fluorescent compound that is enzymatically converted into fluorescent calcein in metabolically active cells. This conversion requires intact membranes and intracellular esterase activity, resulting in strong green fluorescence proportional to viable cell number.

Cytus™ Red selectively stains dead or membrane-compromised cells by intercalating into nucleic acids, producing bright red fluorescence upon binding. Together, these dyes provide a robust and widely validated method for viability assessment across a range of experimental systems.

Parameter, Testing, and Method Cytus™ Green / Red #5471
Live Cell Dye Green (Cytus™ Green Calcein AM)
Dead Cell Dye Red (Cytus™ Red Propidium Iodide)
Stock Concentration Green/Red: 1 mM in DMSO
Stock Volume Green/Red: 100 µL
Appearance Green: yellow hue, Red: red hue; clear liquid
Sterility Non-Sterile
Assay Type Live dead cell viability
Cell Compatibility Live and dead cells
Working Concentration Green: 2 µM; Red: 2 µM
Excitation/Emission (Green) 501 / 521 nm
Excitation/Emission (Red) 537 / 617 nm
Storage Temperature -20°C
Shelf Life Minimum of 6 months from date of receipt

Directions for Use

Directions for Use

Product Applications

  • Cytotoxicity and drug screening assays

  • Live/dead discrimination in 2D and 3D cultures

  • Hydrogel and ECM-based cell viability studies

  • High-content imaging

  • Flow cytometry viability analysis

  • Tissue engineering and bioprinting validation

Product Certificate of Analysis

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Safety and Documentation

Safety Data Sheets

Cytus™ Green

Cytus™ Red

Certificates of Origin

Cytus™ Green

Cytus™ Red

Cytus™ Green Cytus™ Red

Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.