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Collagen
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Type I - Atelocollagen
- PureCol® Solution, 3 mg/ml (bovine) #5005
- Nutragen® Solution, 6 mg/ml (bovine) #5010
- FibriCol® Solution, 10 mg/ml (bovine) #5133
- PureCol® EZ Gel, Solution, 5 mg/ml (bovine) #5074
- PureCol® Lyophilized, 15 mg (bovine) #5006
- VitroCol® Solution, 3 mg/ml (human) #5007
- VitroCol® Lyophilized, 15 mg (human) #5008
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Type I - Telocollagen
- TeloCol®-3 Solution, 3 mg/ml (bovine) #5026
- TeloCol®-6 Solution, 6 mg/ml (bovine) #5225
- TeloCol®-10 Solution, 10 mg/ml (bovine) #5226
- RatCol™ for 2D and 3D, Solution, 4 mg/ml (rat) #5153
- RatCol™ High Concentration, Solution, 10 mg/ml (rat)
- RatCol™ lyophilized, 100 mg (rat)
- RatCol™ for Coatings, Solution, 4 mg/ml (rat) #5056
- Type I - Insoluble Collagen
- Type I - Bioinks
- Type II Collagen
- Type III Collagen
- Type IV Collagen
- Collagen Standard
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PureCol® Collagen Coated Plates
- Collagen Coated T-25 Flasks #5029
- Collagen Coated 6-well Plates #5073
- Collagen Coated 12-well Plates #5439
- Collagen Coated 24-well Plates #5440
- Collagen Coated 48-well Plates #5181
- Collagen Coated 96-well Plates #5072
- Collagen Coated 384-well Plates #5380-5EA
- Collagen Coated 100 x 20 mm Dishes #5028
- MatTek Glass-Bottom Dishes
- MatTek Multi-Well Plates
- Collagen Scaffolds
- Collagen Imaging Reagents
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Type I - Atelocollagen
- Tunable Stiffness
- CytoSoft™ Rigidity Plates
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Bioprinting
- Support Slurry for FRESH Bioprinting
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Bioinks for Extrusion Bioprinting
- Lifeink® 200 Collagen Bioink (35 mg/ml) #5278
- Lifeink® 220 Collagen Bioink (70 mg/ml) #5343
- Lifeink® 240 Acidic Collagen Bioink (35 mg/ml) #5267
- Lifeink® 260 Acidic Collagen Bioink (70 mg/ml) #5358
- GelMA Bioink
- GelMA A Bioink
- GelMA C Bioink
- Pluronic F-127 40% Sterile Solution
- GelMA 20% Sterile Solution
- Alginate 5% Sterile Solution
- Photoinitiators
- Bioinks for BIONOVA X
- Bioinks for Lumen X
- DLP Printing Consumables
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Create Your Own Bioinks
- PhotoCol® Methacrylated Collagen
- PhotoGel® Methacrylated Gelatin 95% DS
- PhotoGel® Methacrylated Gelatin 50% DS
- PhotoHA®-Stiff Methacrylated Hyaluronic Acid
- PhotoHA®-Soft Methacrylated Hyaluronic Acid
- PhotoAlginate® Methacrylated Alginate
- PhotoDextran® Methacrylated Dextran
- PEGDA (Various Molecular Weights)
- Silk Fibroin, Solution
- PhotoSericin® Methacrylated Sericin
- Bioprinters
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3D Hydrogels
- Thermoreversible Hydrogel
- Silk Fibroin
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Type I Collagen for 3D Hydrogels
- PureCol® Solution, 3 mg/ml (bovine) #5005
- Nutragen® Solution, 6 mg/ml (bovine) #5010
- FibriCol® Solution, 10 mg/ml (bovine) #5133
- PureCol® EZ Gel, Solution, 5 mg/ml (bovine) #5074
- VitroCol® Solution, 3 mg/ml (human) #5007
- TeloCol®-3 Solution, 3 mg/ml (bovine) #5026
- TeloCol®-6 Solution, 6 mg/ml (bovine) #5225
- TeloCol®-10 Solution, 10 mg/ml (bovine) #5226
- RatCol® for 3D gels, Solution, 4 mg/ml (rat) #5153
- HyStem® Thiolated Hyaluronic Acid
- Methacrylated Collagen
- Methacrylated Gelatin
- Methacrylated Hyaluronic Acid
- Diacrylates
- Collagen Sponges
- Methacrylated Polysaccharides
- Spheroids and Organoids
- Extracellular Matrices
- HyStem / Hyaluronic Acid
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Adhesion Peptides / Proteins
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Recombinant Adhesion Proteins
- CD2, 0.5 mg/ml #5086
- CDH3, 0.5 mg/ml #5124
- CDH13, 0.5 mg/ml #5125
- CD14, 0.5 mg/ml #5089
- CDH18, 0.5 mg/ml #5090
- CD40, 0.5 mg/ml #5093
- CD86, 0.5 mg/ml #5096
- CD164, 0.5 mg/ml #5100
- CD270, 0.5 mg/ml #5127
- CD274, 0.5 mg/ml #5126
- CD276, 0.5 mg/ml #5123
- E-Cadherin (CD324), 0.5 mg/ml #5085
- ICAM2, 0.5 mg/ml #5107
- Adhesion Peptides
- Collagen Hybridizing Peptides
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Recombinant Adhesion Proteins
- Reagents
- Assays
Cytus™ RGB
Cytus™ RGB Cell Viability + Nuclear Staining Kit (Red/Green/Blue)
Catalog #5470
Cytus™ RGB
Cytus™ RGB Cell Viability + Nuclear Staining Kit (Red/Green/Blue)
Catalog #5470
All-in-one fluorescence kit for cell viability and nuclear staining. Combines Calcein AM, Propidium Iodide, and Hoechst-33342 Cytus™ Blue for simultaneous visualization of viable cells, dead cells, and nuclei. Ideal for imaging, screening, and 3D cell culture workflows.
Product Description
The Cytus™ RGB Cell Viability and Nucleus Stain Kit is a comprehensive, three-color fluorescence assay that enables simultaneous visualization of live cells, dead cells, and nuclei. The kit combines Cytus™ Green (Calcein AM), Cytus™ Red (Propidium Iodide), and Cytus™ Blue (Hoechst 33342) to provide a complete picture of cell health and population structure within a single experiment.
Cytus™ Green labels metabolically active cells through esterase-mediated conversion to fluorescent calcein, while Cytus™ Red selectively stains membrane-compromised cells by binding nucleic acids. Cytus™ Blue binds DNA and provides high-contrast nuclear labeling in both live and fixed cells.
Together, these dyes deliver strong signal separation across green, red, and blue channels, enabling robust qualitative imaging and quantitative analysis across microscopy, plate-based assays, and flow cytometry workflows.
| Parameter, Testing, and Method | Cytus™ RGB #5470 |
| Live Cell Dye | Green (Cytus™ Green Calcein AM) |
| Dead Cell Dye | Red (Cytus™ Red Propidium Iodide) |
| Nuclear Dye | Blue (Cytus™ Blue Hoechst-33342) |
| Stock Concentration | Green/Red: 1 mM in DMSO; Blue: 1 mM in water |
| Stock Volume | Green/Red: 100 µL; Blue: 100 µL |
| Appearance | Green: yellow hue, Red: red hue, Blue: green hue; clear liquid |
| Sterility | Non-Sterile |
| Assay Type | Cell Viability + Nuclear (3-color) |
| Cell Compatibility | Live, dead and fixed cells |
| Working Concentration | Green: 2 µM; Red: 2 µM; Blue: 1–5 µM |
| Excitation/Emission (Green) | 501 / 521 nm |
| Excitation/Emission (Red) | 537 / 617 nm |
| Excitation/Emission Blue | ~350 / 454 nm |
| Storage Temperature | -20°C |
| Shelf Life | Minimum of 6 months from date of receipt |
Product Applications
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Cell viability + nuclear staining in a single assay
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3D cell culture and hydrogel viability analysis
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Drug screening and cytotoxicity studies
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High-content imaging (HCS)
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Cell counting and normalization
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Tissue engineering and bioprinting validation
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ECM interaction studies (collagen, HA, GelMA systems)
Product Certificate of Analysis
No result for .
Safety and Documentation
Safety Data Sheets
Certificates of Origin
Product Disclaimer
This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
