Stiffness Regulates Signaling Pathways
09/21/20
Publication Title:
Cellular Microenvironment Stiffness Regulates Eicosanoid Production and Signaling Pathways
Advanced BioMatrix Products Used:
CytoSoft® PDMS - Soft Substrate Plates
RatCol® Type I Telocollagen (4 mg/ml) from rat tail
Advanced BioMatrix Collagen eBrochure
How the products were used:
CytoSoft® plates with a substrate stiffness of 2 kPa were used to culture fibroblasts and compare to conventional tissue culture on 2D plastic (which has a stiffness of ~3 Gp).
RatCol® collagen was used to coat the CytoSoft® plates.
Abstract:
Our study reveals an extensive dysregulation of prostanoid biosynthesis and signalling pathways in IPF-derived fibroblasts which are only partially abrogated by culture in soft microenvironments.
Link to the publication:
https://www.atsjournals.org/doi/abs/10.1165/rcmb.2020-0227OC
