Stiffness Regulates Mechanosignaling
06/10/21
Publication Title:
Substrate Stiffness and Stretch Regulate Profibrotic Mechanosignaling in Pulmonary Arterial Adventitial Fibroblasts
Advanced BioMatrix Products Used:
CytoSoft® PDMS Soft Substrate Plates
How the products were used:
CytoSoft® Plates (6-well plates with a thin layer of PDMS of a specific stiffness, including 0.2, 0.5, 2, 8, 16, 32 and 64 kPa) were used to show that the expression of collagens I and III and elastin messenger RNAs (Col1a1, Col3a1, Eln) were upregulated by increased substrate stiffness in pulmonary arterial adventitial fibroblasts.
Article Conclusion:
"These novel in-silico models of PAAF profibrotic cell signaling in response to altered mechanical conditions may help identify regulators of vascular adventitial remodeling due to changes in stretch and matrix stiffness that occur during the progression of pulmonary arterial hypertension in-vivo."
Link to the publication:
https://www.mdpi.com/2073-4409/10/5/1000
